HRP Anti-CHREBP antibody (ab81958)
Key features and details
- HRP Rabbit polyclonal to CHREBP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-CHREBP antibody
See all CHREBP primary antibodies -
Description
HRP Rabbit polyclonal to CHREBP -
Host species
Rabbit -
Conjugation
HRP -
Specificity
A customer has observed some cross-reactivity with MLX-interacting protein (Q9HAP2). Although the exact sequence is proprietary, the immunogen for ab81958 shows 75% homology with this protein. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CHREBP aa 800-852 (C terminal). The exact sequence is proprietary.
Database link: NP_116569.1 -
Positive control
- Human hepatocyte lysate, human hepatocytes, liver nuclear extracts from well fed rats, and colon epithelium.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified rabbit antisera. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-CHREBP antibody (ab81958)anti CHREBP (unconjugated form of ab81958) + human hepatocyte lysate at 20 µg
Predicted band size: 95 kDa
Observed band size: 95 kDa
5-10 second film exposure. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CHREBP antibody (ab81958)anti CHREBP (unconjugated form of ab81958), staining CHREBP in human colon epithelium (40X) by immunohistochemistry.
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Immunocytochemistry/ Immunofluorescence - HRP Anti-CHREBP antibody (ab81958)anti CHREBP (unconjugated form of ab81958), at a 1/100 dilution, staining CHREBP in human hepatocyte by Immunofluorescence. Overlay staining of ChREBP (rhodamine red) and DAPI nuclear staining (blue). -
Western blot - HRP Anti-CHREBP antibody (ab81958)All lanes : anti CHREBP (unconjugated form of ab81958), at 1/1000 dilution
Lane 1 : liver nuclear extracts from well fed rats at 10 µg
Lane 2 : liver nuclear extracts from well fed rats at 5 µg
Lane 3 : liver nuclear extracts from well fed rats at 1 µg
Lane 4 : liver nuclear extracts from well fed rats at 0.5 µg
Predicted band size: 95 kDa
Observed band size: 95 kDa
7% SDS gel.
