Anti-Nestin antibody - Neural Stem Cell Marker (ab7659)
Key features and details
- Rabbit polyclonal to Nestin - Neural Stem Cell Marker
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Nestin antibody - Neural Stem Cell Marker
See all Nestin primary antibodies -
Description
Rabbit polyclonal to Nestin - Neural Stem Cell Marker -
Host species
Rabbit -
Specificity
Detects a band at 200kDa representing Nestin in MEFs. Denaturing of WB protein lysate causes loss of Nestin immunoreactivity in WB. -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide:
ALETESQDSAEPSGSEE
, corresponding to amino acids 1481-1497 of Human Nestin. -
General notes
ab7659 has been upgraded from Fast Track status based on data obtained in a new batch of this antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Nestin antibody - Neural Stem Cell Marker (ab7659)Anti-Nestin antibody - Neural Stem Cell Marker (ab7659) at 1/2000 dilution + MEF whole cell lysate at 30 µg
Secondary
IRDye 800
conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 176 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
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Western blot - Anti-Nestin antibody - Neural Stem Cell Marker (ab7659) This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, FranceAll lanes : Anti-Nestin antibody - Neural Stem Cell Marker (ab7659) at 1 µg/ml
Lane 1 : Mouse neural precursors whole cell lysate reduced but not denatured
Lane 2 : Mouse neural precursors whole cell lysate reduced and denatured
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 176 kDa
Observed band size: 230 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes
A slightly higher band obtained than expected, but as predicted in the datasheet. Denaturing of the WB lysate results in a loss of Nestin specific immunoreactivity. NB: Equal protein loading was verified.
