Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (ab247623)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1529] to alpha Internexin - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free
    See all alpha Internexin primary antibodies

  • Description

    Rabbit monoclonal [EPR1529] to alpha Internexin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247623 is the carrier-free version of ab108302. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247623 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (ab247623)
    Western blot - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (ab247623)
    All lanes : Anti-alpha Internexin antibody [EPR1529] (ab108302) at 1/10000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : Y79 cell lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Performed under non-reducing conditions.

    Predicted band size: 55 kDa



    This data was developed using ab108302, the same antibody clone in a different buffer formulation.

    Secondary antibody - goat anti-rabbit HRP (ab6721).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (ab247623)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (ab247623)
    This data was developed using ab108302, the same antibody clone in a different buffer formulation.ab108302, at 1/250, staining alpha Internexin in human brain tissue by immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (ab247623)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (ab247623)
    This data was developed using ab108302, the same antibody clone in a different buffer formulation.ab108302 showing negative staining in Normal human colon tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (ab247623)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (ab247623)
    This data was developed using ab108302, the same antibody clone in a different buffer formulation.ab108302 showing negative staining in Normal human tonsil tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (ab247623)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (ab247623)
    This data was developed using ab108302, the same antibody clone in a different buffer formulation.ab108302 showing negative staining in Normal human liver tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (ab247623)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (ab247623)
    This data was developed using ab108302, the same antibody clone in a different buffer formulation.ab108302 showing negative staining in Normal human breast tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (ab247623)
    Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (ab247623)

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