Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23656-5] to A2BP1/Fox1/RBFOX1 - BSA and Azide free
  • Suitable for: IHC-Fr, WB, IHC-P, Flow Cyt (Intra), ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free
    See all A2BP1/Fox1/RBFOX1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23656-5] to A2BP1/Fox1/RBFOX1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC
    Mouse
    IHC-Fr
    Mouse
    IHC-P
    Rat
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain cortex, brain and skeletal muscle; Rat brain and brain cortex. IHC-P: Human cerebrum and skeletal muscle; Rat cerebrum; Mouse cerebrum. IHC-Fr: Mouse cerebrum, Rat cerebrum ICC: Mouse primary neural/glia and rat primary neural/glia, Neuro-2a cells. Flow Cyt: Mouse primary neuron cells.

  • General notes

    ab278527 is the carrier-free version of ab254413. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab278527 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)

    This data was developed using ab254413, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling A2BP1/Fox1/RBFOX1 with ab254413 at 1/2000 (0.239 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human skeletal muscle. The section was incubated with ab254413 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)

    This data was developed using ab254413, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling A2BP1/Fox1/RBFOX1 with ab254413 at 1/2000 (0.239 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human brain. The section was incubated with ab254413 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
    Western blot - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
    All lanes : Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (ab254413) at 1/1000 dilution

    Lane 1 : Mouse brain cortex tissue lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Mouse skeletal muscle tissue lysate
    Lane 4 : Mouse liver tissue lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat brain cortex tissue lysate
    Lane 7 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 42 kDa



    This data was developed using ab254413, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: liver (PMID:12574126, PMID:16260614, PMID:20724578, PMID:20724578, PMID:10814712).

    Exposure time: 37 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)

    This data was developed using ab254413, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling A2BP1/Fox1/RBFOX1 with ab254413 at 1/2000 (0.239 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum. The section was incubated with ab254413 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
    Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)

    This data was developed using ab254413, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling A2BP1/Fox1/RBFOX1 with ab254413 at 1/100 (4.78 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunocytochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
    Immunocytochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)

    This data was developed using ab254413, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling A2BP1/Fox1/RBFOX1 with ab254413 at 1/50 (9.56 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear and cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunocytochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
    Immunocytochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)

    This data was developed using ab254413, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a cells labelling A2BP1/Fox1/RBFOX1 with ab254413 at 1/50 (9.56 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in Neuro-2a cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
    Flow Cytometry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)

    This data was developed using ab254413, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells cells labelling A2BP1/Fox1/RBFOX1 with ab254413 at 1/1000 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)

    This data was developed using ab254413, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling A2BP1/Fox1/RBFOX1 with ab254413 at 1/2000 (0.239 ug/ml) dilution  followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebrum. The section was incubated with ab254413 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
    Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)

    This data was developed using ab254413, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum tissue labeling A2BP1/Fox1/RBFOX1 with ab254413 at 1/100 (4.78 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunocytochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
    Immunocytochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)

    This data was developed using ab254413, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling A2BP1/Fox1/RBFOX1 with ab254413 at 1/50 (9.56 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

     

  • Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
    Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)

    This data was developed using ab254413, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver tissue labeling A2BP1/Fox1/RBFOX1 with ab254413 at 1/100 (4.78 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Negative control: no staining on mouse liver (PMID: 16260614) is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
    Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)

    This data was developed using ab254413, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver tissue labeling A2BP1/Fox1/RBFOX1 with ab254413 at 1/100 (4.78 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Negative control: no staining on rat liver (PMID: 16260614) is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)

    This data was developed using ab254413, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling A2BP1/Fox1/RBFOX1 with ab254413 at 1/2000 (0.239 ug/ml) dilution  followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on human liver (PMID:10814712). The section was incubated with ab254413 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)

    This data was developed using ab254413, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling A2BP1/Fox1/RBFOX1 with ab254413 at 1/2000 (0.239 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on mouse liver. The section was incubated with ab254413 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)

    This data was developed using ab254413, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling A2BP1/Fox1/RBFOX1 with ab254413 at 1/2000 (0.239 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on rat liver. The section was incubated with ab254413 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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