Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Slit2 antibody [EPR23272-227] - BSA and Azide free (ab275092)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23272-227] to Slit2 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Slit2 antibody [EPR23272-227] - BSA and Azide free
    See all Slit2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23272-227] to Slit2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: rat brain tissue, PC-3, T-47, mIMCD3, Mouse E14.5 brain tissue, 293T.

  • General notes

    ab275092 is the carrier-free version of ab246503. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275092 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Slit2 antibody [EPR23272-227] - BSA and Azide free (ab275092)
    Western blot - Anti-Slit2 antibody [EPR23272-227] - BSA and Azide free (ab275092)
    Anti-Slit2 antibody [EPR23272-227] (ab246503) at 1/1000 dilution + Rat brain tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG (H+L), Peroxidase conjugated)

    Predicted band size: 169 kDa



    This data was developed using ab246503, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The antibody detects Slit2-full(200KDa) and Slit2-N fragment (150KDa).

    The molecular weight observed is consistent with what has been described in the literature (PMID: 11404413).

    Exposure time: 3 minutes.

     

  • Western blot - Anti-Slit2 antibody [EPR23272-227] - BSA and Azide free (ab275092)
    Western blot - Anti-Slit2 antibody [EPR23272-227] - BSA and Azide free (ab275092)
    All lanes : Anti-Slit2 antibody [EPR23272-227] (ab246503) at 1/1000 dilution

    Lane 1 : PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate at 20 µg
    Lane 2 : T-47D(Human ductal breast epithelial tumor epithelial cell), whole cell lysate at 20 µg
    Lane 3 : mIMCD3 (mouse inner medlary collecting duct epithelial cell), whole cell lysate at 20 µl
    Lane 4 : Mouse E14.5 brain tissue lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 169 kDa



    This data was developed using ab246503, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    A~25 kDa degraded band is observed. Freshly made lysates can decrease the degradation.

    The antibody detects Slit2-full(200KDa) and Slit2-N fragment (150KDa).

    The molecular weight observed is consistent with what has been described in the literature (PMID: 11404413).

    Negative control: T-47D (PMID:17268810).

    Exposure time: 

    Lanes 1-2: 136 seconds;

    Lanes 3-4: 26 seconds.

     

  • Western blot - Anti-Slit2 antibody [EPR23272-227] - BSA and Azide free (ab275092)
    Western blot - Anti-Slit2 antibody [EPR23272-227] - BSA and Azide free (ab275092)
    All lanes : Anti-Slit2 antibody [EPR23272-227] (ab246503) at 1/1000 dilution

    All lanes : 293T (human embryonic kidney epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 169 kDa



    This data was developed using ab246503, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Fresh lysate was used in lane 1.

    Band detected around 25KDa in lane 2 is caused by degradation as it disappeared in fresh lysate.

    Exposure time: 37 seconds

  • Anti-Slit2 antibody [EPR23272-227] - BSA and Azide free (ab275092)
    Anti-Slit2 antibody [EPR23272-227] - BSA and Azide free (ab275092)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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