Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
![Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)](https://www.abcam.com/ps/products/109/ab109236/Images/ab109236-251526-anti-neurogenin-2-antibody-epr4177-western-blot.jpg "Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4177] to Neurogenin 2/NGN2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Neurogenin 2/NGN2 antibody [EPR4177]
See all Neurogenin 2/NGN2 primary antibodies -
Description
Rabbit monoclonal [EPR4177] to Neurogenin 2/NGN2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB RatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SH-SY5Y and HepG2 cell lysates. ICC/IF: SH-SY-5Y cell lysates.
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General notes
This product was previously labelled as Neurogenin 2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4177 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)All lanes : Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236) at 1/5000 dilution (purified)
Lane 1 : PC-12 cell lysate
Lane 2 : NIH/3T3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 29 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
![Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)](https://www.abcam.com/ps/products/109/ab109236/Images/ab109236-251525-anti-neurogenin-2-antibody-epr4177-western-blot.jpg)
Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236) at 1/5000 dilution (purified) + SH-SY5Y cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 29 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
![Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)](https://www.abcam.com/ps/products/109/ab109236/Images/ab109236-121910-anti-neurogenin-2-antibody-epr4177-western-blot.jpg)
Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)All lanes : Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236) at 1/1000 dilution (unpurified)
Lane 1 : SH-SY5Y cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 29 kDa
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![Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)](https://www.abcam.com/ps/products/109/ab109236/Images/ab109236-5-benefits-of-recombinant-antibodies.png)
Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
