Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (ab250225)
![Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (ab250225)](https://www.abcam.com/ps/products/250/ab250225/Images/ab250225-408393-anti-zinc-alpha-2-glycoprotein-antibody-epr14425-b-bsa-and-azide-free-western-blot.jpg "Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (ab250225)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14425(B)] to Zinc Alpha 2 Glycoprotein - BSA and Azide free
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free
See all Zinc Alpha 2 Glycoprotein primary antibodies -
Description
Rabbit monoclonal [EPR14425(B)] to Zinc Alpha 2 Glycoprotein - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250225 is the carrier-free version of ab180574. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250225 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14425(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (ab250225)Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/20000 dilution + Human plasma at 20 µg
Secondary
Anti-rabbit (HRP) specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 34 kDaThis data was developed using ab180574, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (ab250225)](https://www.abcam.com/ps/products/250/ab250225/Images/ab250225-405697-anti-zinc-alpha-2-glycoprotein-antibody-epr14425-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (ab250225)Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/2000 dilution + HepG2 cell lysate at 10 µg
Secondary
Goat Anti-rabbit HRP at 1/1000 dilution
Predicted band size: 34 kDaThis data was developed using ab180574, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (ab250225)](https://www.abcam.com/ps/products/250/ab250225/Images/ab250225-403539-anti-zinc-alpha-2-glycoprotein-antibody-epr14425-b-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (ab250225)This data was developed using ab180574, the same antibody clone in a different buffer formulation.
Immunoprecipitation of Human plasma labeling Zinc Alpha 2 Glycoprotein using ab180574at a 1/30 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes : Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/30 dilution
Lane 1 : Human plasma
Lane 2 : Negative control
Secondary
All lanes : Anti-Rabbit (HRP) specific to the non-reduced form of IgG at 1/500 dilution
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (ab250225)](https://www.abcam.com/ps/products/250/ab250225/Images/ab250225-1-anti-zinc-alpha-2-glycoprotein-antibody-epr14425-b-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (ab250225)This data was developed using ab180574, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Human liver tissue labeling Zinc Alpha 2 Glycoprotein with ab180574 at 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. -
![Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (ab250225)](https://www.abcam.com/ps/products/250/ab250225/Images/ab250225-5-benefits-of-recombinant-antibodies.png)
Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (ab250225)
