Mouse Progranulin ELISA Kit (ab213473)
Key features and details
- One-wash 90 minute protocol
- Range: 93.73 pg/ml - 6000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Progranulin ELISA Kit
See all Granulin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.3% Inter-assay Sample n Mean SD CV% Serum 3 6.6% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
7.8 -
Range
93.73 pg/ml - 6000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 116 115% - 118% Cell culture extracts 107 102% - 109% Cell culture media 93 93% - 95% Hep Plasma 111 107% - 118% EDTA Plasma 115 114% - 118% Cit plasma 115 110% - 119% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse Progranulin ELISA Kit (ab213473) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Progranulin protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Progranulin with 7.8 NULL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Mouse Progranulin belongs to the granulin family and is 572 amino acids (aa) in length. Additionally, mouse Progranulin is cleaved into individual granulin peptides (Granulin-1 through Granulin-7). Granulins have cytokine-like activity and may also play a role in inflammation, wound repair, and tissue remodelling. They also act as an autocrine growth factor for PC cells. Human and rat Progranulin have 75% and 87% sequence homology with mouse Progranulin, respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Progranulin Capture Antibody 1 x 600µl 10X Mouse Progranulin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Mouse Progranulin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.
Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth. -
Tissue specificity
In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney. -
Involvement in disease
Defects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD) [MIM:607485]; also known as tau-negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease. -
Sequence similarities
Belongs to the granulin family. -
Post-translational
modificationsGranulins are disulfide bridged. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Acrogranin
- CLN11
- GEP
see all -
Database links
- Entrez Gene: 14824 Mouse
- SwissProt: P28798 Mouse
- Unigene: 1568 Mouse
Images
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Other - Mouse Progranulin ELISA Kit (ab213473)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Progranulin standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse Progranulin standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Progranulin in mouse serum and plasma samples.The concentrations of Progranulin were measured in duplicates, interpolated from the Progranulin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:250, plasma (citrate) 1:250, plasma (EDTA) 1:250, and plasma (heparin) 1:250. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Progranulin concentration was determined to be 1,610.5 ng/mL in serum, 1,149.5 ng/mL in plasma (citrate), 1,253.7 ng/mL in plasma (EDTA) and 1,710.8 ng/mL in plasma (heparin).
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Interpolated concentrations of native Progranulin in mouse J774A.1 and RAW264.7 cultured media samples and spiked Progranulin in RPMI culture media.The concentrations of Progranulin were measured in duplicates, interpolated from the Progranulin standard curves and corrected for sample dilution. Undiluted samples are as follows: J774A.1 cultured media 1:400, RAW264.7 cultured media 6.25%, and RPMI culture media 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Progranulin concentration was determined to be 197.4 ng/mL in J774A.1 cultured media, 38.4 ng/mL in RAW264.7 cultured media, and 82.6 ng/mL in RPMI culture media. J774A.1 samples were cultured in high glucose DMEM culture media with kanamycin and 10% fetal bovine serum for 72 hours and then stimulated with 1.5% PHA plus 10 ng/mL PMA for 72 hours. RAW264.7 samples were cultured in high glucose DMEM culture media with kanamycin and L‑Glutamine for 72 hours and then stimulated with 5 µg/mL of LPS for 48 hours.
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Interpolated concentrations of native Progranulin in mouse J774A.1 cell extract based on a 12.5 µg/mL extract load.The concentrations of Progranulin were measured in duplicate and interpolated from the Progranulin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Progranulin concentration in J774A.1 cell extract was determined to be 2.9 ng/mL.
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Interpolated concentrations of native Progranulin in mouse J774A.1 cultured media samples.All samples were diluted 1:400 and the concentrations of Progranulin were measured in duplicates, interpolated from the Progranulin standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Progranulin concentration was determined to be 34.8 ng/mL in unstimulated J774A.1 cultured media and 206.9 ng/mL in stimulated J774A.1 cultured media. J774A.1 samples were cultured in high glucose DMEM culture media with kanamycin and 10% fetal bovine serum for 72 hours (unstimulated samples) and then stimulated with 1.5% PHA plus 10 ng/mL PMA for 72 hours.
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Sandwich ELISA - Mouse Progranulin ELISA Kit (ab213473)To learn more about the advantages of recombinant antibodies see here.
