Anti-PKC theta/PRKCQ (phospho S695) antibody (ab227200)
Key features and details
- Rabbit polyclonal to PKC theta/PRKCQ (phospho S695)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-PKC theta/PRKCQ (phospho S695) antibody
See all PKC theta/PRKCQ primary antibodies -
Description
Rabbit polyclonal to PKC theta/PRKCQ (phospho S695) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide within Human PKC theta/PRKCQ (phospho S695). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: Q04759 -
Positive control
- WB: MCF7 (treated with 500 nM PMA), HeLa, HelpG2 and MOLT-4 whole cell lysate.
-
General notes
This product was previously labelled as PKC theta
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-PKC theta/PRKCQ (phospho S695) antibody (ab227200)All lanes : Anti-PKC theta/PRKCQ (phospho S695) antibody (ab227200) at 1/500 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell extract
Lane 2 : MCF7, treated with 500 nM PMA for 1 hour, whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 82 kDa7.5% SDS-PAGE
-
Western blot - Anti-PKC theta/PRKCQ (phospho S695) antibody (ab227200)All lanes : Anti-PKC theta/PRKCQ (phospho S695) antibody (ab227200) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 82 kDa7.5% SDS PAGE
