Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PPP2R2B antibody [EPR10961] - BSA and Azide free (ab249330)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10961] to PPP2R2B - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PPP2R2B antibody [EPR10961] - BSA and Azide free
    See all PPP2R2B primary antibodies

  • Description

    Rabbit monoclonal [EPR10961] to PPP2R2B - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The antibody may also recognize PPP2R2A and PPP2R2D based on our internal QC data.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249330 is the carrier-free version of ab157461. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249330 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PPP2R2B antibody [EPR10961] - BSA and Azide free (ab249330)
    Western blot - Anti-PPP2R2B antibody [EPR10961] - BSA and Azide free (ab249330)
    Anti-PPP2R2B antibody [EPR10961] (ab157461) at 1/1000 dilution + GST-tagged Recombinant Human PPP2R2B protein (aa 1 to 443) 15ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 52 kDa
    Observed band size: 76 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab157461, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3.25 seconds.

    Recombinant Human PPP2R2B protein (Cat

    : ab132644).

  • Western blot - Anti-PPP2R2B antibody [EPR10961] - BSA and Azide free (ab249330)
    Western blot - Anti-PPP2R2B antibody [EPR10961] - BSA and Azide free (ab249330)
    All lanes : Anti-PPP2R2B antibody [EPR10961] (ab157461) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : Human fetal brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 52 kDa



    This data was developed using ab157461, the same antibody clone in a different buffer formulation.

     

  • Anti-PPP2R2B antibody [EPR10961] - BSA and Azide free (ab249330)
    Anti-PPP2R2B antibody [EPR10961] - BSA and Azide free (ab249330)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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