Anti-PPP2R2B antibody (ab264160)
Key features and details
- Rabbit polyclonal to PPP2R2B
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PPP2R2B antibody
See all PPP2R2B primary antibodies -
Description
Rabbit polyclonal to PPP2R2B -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow, Pig, Orangutan
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Immunogen
Synthetic peptide within Human PPP2R2B aa 25-75. The exact sequence is proprietary. NP_004567.1
Database link: Q00005 -
Positive control
- WB: HeLa, HEK-293T and TCMK whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PPP2R2B antibody (ab264160)All lanes : Anti-PPP2R2B antibody (ab264160) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : TCMK (Mouse kidney epithelial cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Predicted band size: 52 kDa
Exposure time: 3 minutes4-20% SDS-PAGE
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Immunoprecipitation - Anti-PPP2R2B antibody (ab264160)PPP2R2B was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264160 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab264160 at 0.4 µg/ml.
Lane 1: ab264160 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 10 secs.
Cells prepared using NETN lysis buffer.
