Anti-PPP1A/PPP1CA (phospho T320) antibody [EP1512Y] (ab62334)
![Anti-PPP1A/PPP1CA (phospho T320) antibody [EP1512Y] (ab62334)](https://www.abcam.com/ps/products/62/ab62334/Images/ab62334-75637-anti-ppp1a-phospho-t320-antibody-ep1512y-western-blot.jpg "Anti-PPP1A/PPP1CA (phospho T320) antibody [EP1512Y] (ab62334)")
Key features and details
- Rabbit monoclonal [EP1512Y] to PPP1A/PPP1CA (phospho T320)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PPP1A/PPP1CA (phospho T320) antibody [EP1512Y]
See all PPP1A/PPP1CA primary antibodies -
Description
Rabbit monoclonal [EP1512Y] to PPP1A/PPP1CA (phospho T320) -
Host species
Rabbit -
Specificity
This antibody detects PP1 alpha phosphorylated at Threonine 320.
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Xenopus laevis
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa cell lysate. IHC-P: Human muscle tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1512Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PPP1A/PPP1CA (phospho T320) antibody [EP1512Y] (ab62334)All lanes : Anti-PPP1A/PPP1CA (phospho T320) antibody [EP1512Y] (ab62334)
Lane 1 : HeLa cell lysate (untreated)
Lane 2 : HeLa cell lysate (treated with Calyculin A)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
The bottom image shows beta Tubulin as a positive control. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1A/PPP1CA (phospho T320) antibody [EP1512Y] (ab62334)](https://www.abcam.com/ps/products/62/ab62334/Images/ab62334-75636-anti-ppp1a-phospho-t320-antibody-ep1512y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1A/PPP1CA (phospho T320) antibody [EP1512Y] (ab62334)Ab62334 (1/100-1/250) staining human PPP1A/PPP1CA in human muscle tissue by immunohistochemistry using paraffin embedded tissue.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
