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Signal Transduction Protein Phosphorylation Ser / Thr Phosphatases

Anti-PPP1A/PPP1CA antibody [1C11-C10-H5-E9] (ab150782)

Anti-PPP1A/PPP1CA antibody [1C11-C10-H5-E9] (ab150782)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [1C11-C10-H5-E9] to PPP1A/PPP1CA
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-PPP1A/PPP1CA antibody [1C11-C10-H5-E9]
    See all PPP1A/PPP1CA primary antibodies
  • Description

    Mouse monoclonal [1C11-C10-H5-E9] to PPP1A/PPP1CA
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human PPP1A/PPP1CA. Expressed in E.coli
    Database link: NM_001008709

  • Positive control

    • A431, HeLa and Jurkat Whole cell lysates, Human breast cancer tissue, HeLa cells
  • General notes

    This product was changed from ascites to tissue culture supernatant on 17.08.2018.  Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

     This product was previously labelled as PPP1A

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin 300
    Constituent: 50% Glycerol
  • Concentration information loading...
  • Purity

    Concentrated Culture Supernatant
  • Clonality

    Monoclonal
  • Clone number

    1C11-C10-H5-E9
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Phosphatases
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism

Images

  • Western blot - Anti-PPP1A/PPP1CA antibody [1C11-C10-H5-E9] (ab150782)
    Western blot - Anti-PPP1A/PPP1CA antibody [1C11-C10-H5-E9] (ab150782)
    All lanes : Anti-PPP1A/PPP1CA antibody [1C11-C10-H5-E9] (ab150782) at 1/1000 dilution

    Lane 1 : K562 cell lysates
    Lane 2 : 3T3 cell lysates
    Lane 3 : Hela cell lysates

    Predicted band size: 37 kDa

  • Western blot - Anti-PPP1A/PPP1CA antibody [1C11-C10-H5-E9] (ab150782)
    Western blot - Anti-PPP1A/PPP1CA antibody [1C11-C10-H5-E9] (ab150782)
    All lanes : Anti-PPP1A/PPP1CA antibody [1C11-C10-H5-E9] (ab150782) at 1/1000 dilution

    Lane 1 : A431 whole cell lysates
    Lane 2 : Jurkat whole cell lysates
    Lane 3 : HeLa whole cell lysates

    Predicted band size: 37 kDa



    This image was produced with antibody produced in ascites. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1A/PPP1CA antibody [1C11-C10-H5-E9] (ab150782)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1A/PPP1CA antibody [1C11-C10-H5-E9] (ab150782)

    Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue, labelling PPP1A/PPP1CA using ab150782 at a 1/500 dilution. This image was produced with antibody produced in ascites. 

  • Immunocytochemistry/ Immunofluorescence - Anti-PPP1A/PPP1CA antibody [1C11-C10-H5-E9] (ab150782)
    Immunocytochemistry/ Immunofluorescence - Anti-PPP1A/PPP1CA antibody [1C11-C10-H5-E9] (ab150782)

    Immunocytochemistry analysis of HeLa cells, labelling PPP1A/PPP1CA using ab150782 at a 1/300 dilution. This image was produced with antibody produced in ascites. 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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