Anti-PPP1A/PPP1CA antibody (ab137512)
Key features and details
- Rabbit polyclonal to PPP1A/PPP1CA
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-PPP1A/PPP1CA antibody
See all PPP1A/PPP1CA primary antibodies -
Description
Rabbit polyclonal to PPP1A/PPP1CA -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Xenopus laevis, Xenopus tropicalis
-
Immunogen
Recombinant fragment corresponding to Human PPP1A/PPP1CA aa 1-274.
-
Positive control
- 293T and A431 whole cell lysates; PPP1A/PPP1CA siRNA; paraformaldehyde fixed HeLa cells; Human SAS xenograft tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-PPP1A/PPP1CA antibody (ab137512)All lanes : Anti-PPP1A/PPP1CA antibody (ab137512) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa
12% SDS PAGE -
Western blot - Anti-PPP1A/PPP1CA antibody (ab137512)All lanes : Anti-PPP1A/PPP1CA antibody (ab137512) at 1/1000 dilution
Lane 1 : U2OS cells treated with control siRNA
Lane 2 : U2OS cells treated with PP1A/PPP1CA siRNA
Predicted band size: 37 kDa
Nuclear matrix protein p84 is a loading control, blotted with a p84 antibody at a 1/5000 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-PPP1A/PPP1CA antibody (ab137512)Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa cells (green) labelling PPP1A/PPP1CA with ab137512 at 1/500 dilution. Alpha tubulin filaments were labeled with an Alpha tubulin antibody (red) at 1/2500 dilution.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1A/PPP1CA antibody (ab137512)Immunohistochemical analysis of paraffin embedded Human SAS xenograft tissue labelling PPP1A/PPP1CA with ab137512 at 1/100 dilution.
