Anti-PPM1D/WIP1 antibody (ab31270)
Key features and details
- Rabbit polyclonal to PPM1D/WIP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PPM1D/WIP1 antibody
See all PPM1D/WIP1 primary antibodies -
Description
Rabbit polyclonal to PPM1D/WIP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
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Positive control
- This antibody gave a positive signal in the following whole cell lysates: Jurkat and HEK293 in WB.
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General notes
This product was previously labelled as PPM1D
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab31270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatXenopus laevisZebrafishApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).Target
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Function
Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. -
Sequence similarities
Belongs to the PP2C family.
Contains 1 PP2C-like domain. - Information by UniProt
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Database links
- Entrez Gene: 8493 Human
- Entrez Gene: 53892 Mouse
- Entrez Gene: 380383 Xenopus laevis
- Entrez Gene: 394065 Zebrafish
- Omim: 605100 Human
- SwissProt: O15297 Human
- SwissProt: Q9QZ67 Mouse
- Unigene: 286073 Human
see all -
Alternative names
- EC 3.1.3.16 antibody
- p53 induced protein phosphatase 1 antibody
- p53-induced protein phosphatase 1 antibody
see all
Images
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Western blot - Anti-PPM1D/WIP1 antibody (ab31270)All lanes : Anti-PPM1D/WIP1 antibody (ab31270) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 20 minutes
Protocols
Datasheets and documents
References (8)
ab31270 has been referenced in 8 publications.
- Deng K et al. WIP1 promotes cancer stem cell properties by inhibiting p38 MAPK in NSCLC. Signal Transduct Target Ther 5:36 (2020). PubMed: 32296033
- Lu ZW et al. Silencing of PPM1D inhibits cell proliferation and invasion through the p38 MAPK and p53 signaling pathway in papillary thyroid carcinoma. Oncol Rep 43:783-794 (2020). PubMed: 31922231
- Gong H et al. APPBP2 enhances non-small cell lung cancer proliferation and invasiveness through regulating PPM1D and SPOP. EBioMedicine 44:138-149 (2019). PubMed: 31105033
- Wang P et al. Wip1 cooperates with KPNA2 to modulate the cell proliferation and migration of colorectal cancer via a p53-dependent manner. J Cell Biochem 120:15709-15718 (2019). PubMed: 31127650
- Long X & Lin XJ P65-mediated miR-590 inhibition modulates the chemoresistance of osteosarcoma to doxorubicin through targeting wild-type p53-induced phosphatase 1. J Cell Biochem N/A:N/A (2018). PubMed: 30387173
- Wang ZP et al. Wild-type p53-induced phosphatase 1 is a prognostic marker and therapeutic target in bladder transitional cell carcinoma. Oncol Lett 13:875-880 (2017). PubMed: 28356972
- Li GB et al. Protein phosphatase magnesium-dependent 1d (PPM1D) mRNA expression is a prognosis marker for hepatocellular carcinoma. PLoS One 8:e60775 (2013). IHC-P ; Human . PubMed: 23556002
- Zajkowicz A et al. Nutlin-3a, an MDM2 antagonist and p53 activator, helps to preserve the replicative potential of cancer cells treated with a genotoxic dose of resveratrol. Mol Biol Rep 40:5013-26 (2013). WB ; Human . PubMed: 23666059
Images
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Western blot - Anti-PPM1D/WIP1 antibody (ab31270)All lanes : Anti-PPM1D/WIP1 antibody (ab31270) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 20 minutes
