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Signal Transduction Protein Phosphorylation Ser / Thr Phosphatases

Anti-PPM1D/WIP1 antibody [OTI3E1] (ab236515)

Anti-PPM1D/WIP1 antibody [OTI3E1] (ab236515)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [OTI3E1] to PPM1D/WIP1
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-PPM1D/WIP1 antibody [OTI3E1]
    See all PPM1D/WIP1 primary antibodies
  • Description

    Mouse monoclonal [OTI3E1] to PPM1D/WIP1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human PPM1D/WIP1 aa 1-400. Produced in E.coli (NP_003611).
    Database link: O15297

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: pCMV6-ENTRY PPM1D/WIP1 cDNA transfected HEK-293T cell lysate; HeLa and HEK-293T cell lysates.
  • General notes

     This product was previously labelled as PPM1D

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified from cell culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    OTI3E1
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Phosphatases
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Phosphatases

Images

  • Western blot - Anti-PPM1D/WIP1 antibody [OTI3E1] (ab236515)
    Western blot - Anti-PPM1D/WIP1 antibody [OTI3E1] (ab236515)
    All lanes : Anti-PPM1D/WIP1 antibody [OTI3E1] (ab236515) at 1/2000 dilution

    Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : pCMV6-ENTRY PPM1D/WIP1 cDNA transfected HEK-293T cell lysate

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 67 kDa

  • Western blot - Anti-PPM1D/WIP1 antibody [OTI3E1] (ab236515)
    Western blot - Anti-PPM1D/WIP1 antibody [OTI3E1] (ab236515)
    Anti-PPM1D/WIP1 antibody [OTI3E1] (ab236515) at 1/500 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 35 µg

    Predicted band size: 67 kDa

  • Western blot - Anti-PPM1D/WIP1 antibody [OTI3E1] (ab236515)
    Western blot - Anti-PPM1D/WIP1 antibody [OTI3E1] (ab236515)
    Lanes 1-2 : Anti-PPM1D/WIP1 antibody [OTI3E1] (ab236515) at 1/200 dilution
    Lanes 3-4 : Anti-beta-Actin

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : PPM1D.WIP1-Knockout HEK-293T cell lysate
    Lane 3 : HEK-293T cell lysate
    Lane 4 : PPM1D/WIP1-Knockout HEK-293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 67 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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