Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PHLPP2 antibody [SP220] (ab227673)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP220] to PHLPP2
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-PHLPP2 antibody [SP220]
    See all PHLPP2 primary antibodies

  • Description

    Rabbit monoclonal [SP220] to PHLPP2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human prostate, and prostate carcinoma tissue; WB: HEK293T cell lysate.

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] (ab227673)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] (ab227673)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate carcinoma tissue sections labeling PHLPP2 with ab227673 at 1/100 dilution (1.65 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] (ab227673)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] (ab227673)

    Formalin-fixed, paraffin-embedded human prostate tissue stained for PHLPP2 using ab227673 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] (ab227673)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] (ab227673)

    Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PHLPP2 using ab227673 at 1/100 dilution in immunohistochemical analysis.

  • Western blot - Anti-PHLPP2 antibody [SP220] (ab227673)
    Western blot - Anti-PHLPP2 antibody [SP220] (ab227673)
    Anti-PHLPP2 antibody [SP220] (ab227673) at 1/400 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate.

    Predicted band size: 147 kDa

  • Anti-PHLPP2 antibody [SP220] (ab227673)
    Anti-PHLPP2 antibody [SP220] (ab227673)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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