Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Signal Transduction Protein Phosphorylation Ser / Thr Phosphatases

Human PPP1R2 (Protein phosphatase 1 inhibitor subunit 2) knockout HeLa cell lysate (ab259057)

Price and availability

318 288 ₸

Availability

Order now and get it on Friday March 05, 2021

Human PPP1R2 (Protein phosphatase 1 inhibitor subunit 2) knockout HeLa cell lysate (ab259057)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

You may also be interested in

Product image
Anti-PPM1D/WIP1 antibody [OTI3E1] (ab236515)
Product image
Anti-PP2A-alpha antibody - BSA and Azide free (Capture) (ab242579)
Product image
Anti-DUSP9 antibody (ab167080)
Product image
Anti-PPP2R3B antibody (ab72026)

Overview

  • Product name

    Human PPP1R2 (Protein phosphatase 1 inhibitor subunit 2) knockout HeLa cell lysate
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HeLa
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon1.
  • Passage number

  • Knockout validation

    Sanger Sequencing
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab262609 - Human PPP1R2 knockout HeLa cell lysate 1 x 100µg
    ab255929 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Phosphatases
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10

Target

  • Function

    Inhibitor of protein-phosphatase 1.
  • Sequence similarities

    Belongs to the protein phosphatase inhibitor 2 family.
  • Post-translational
    modifications

    Phosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex (By similarity). Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex.
  • Target information above from: UniProt accession P41236 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • IPP 2
    • IPP-2
    • IPP2
    • IPP2_HUMAN
    • MGC87148
    • Phosphatase inhibitor 2
    • Phosphoprotein phosphatase
    • Ppp1r2
    • PPP1R2 protein
    • Protein phosphatase 1 regulatory (inhibitor) subunit 2
    • Protein phosphatase 1 regulatory subunit 2
    • Protein phosphatase inhibitor 2
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab262609 - Human PPP1R2 knockout HeLa cell lysate 1 x 100µg
    ab255929 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Phosphatases
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10

Images

  • Sanger Sequencing - Human PPP1R2 knockout HeLa cell lysate (ab259057)
    Sanger Sequencing - Human PPP1R2 knockout HeLa cell lysate (ab259057)
    Homozygous: Insertion of the selection cassette in exon1

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

  •  
  • Product image

    Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] - BSA and Azide free (ab247360)

  •  
  • Product image

    Anti-KIR2DL1 + KIR2DL3 + KIR2DL4 + KIR2DS4 antibody (ab197927)

  •  
  • Recombinant mouse KGF protein (Active) (ab200290)

  •  
  • Product image

    Recombinant Human CRTAP/CASP protein (denatured) (ab177624)

  •  
  • Product image

    Recombinant Human Parathyroid Hormone Receptor 1/PTH1R protein (His tag) (ab182670)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.