Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

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Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22960-39] to PPM1D/WIP1
Suitable for: ICC/IF, Flow Cyt, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-PPM1D/WIP1 antibody [EPR22960-39]
See all PPM1D/WIP1 primary antibodies
Description

Rabbit monoclonal [EPR22960-39] to PPM1D/WIP1
Host species

Rabbit
Specificity

The antibody detects specifically PPM1D430.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MCF7, Jurkat, A549, F9, HCT 116, RAW 264.7, PC-12 and NIH/3T3 whole cell lysate, Human testis and lung cancer tissue lysates, Mouse and Rat spleen tissue lysates. ICC/IF: MCF7, HCT-116 cells. Flow Cyt: MCF7 cell.
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies.

For details on our patents, please refer to www.abcam.com/RabMAbPatents
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22960-39
Isotype

IgG
Research areas

Western blot - Anti-Secretory stress proteins (SSP) antibody [EPR22960-39] (ab234439)

All lanes : Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439) at 1/1000 dilution

Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell line) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 4 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate
Lane 5 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 8 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Observed band size: 50 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Exposure time: Lanes 1-4: 70 seconds; Lanes 5-8: 48 seconds.
Immunocytochemistry/ Immunofluorescence - Anti-Secretory stress proteins (SSP) antibody [EPR22960-39] (ab234439)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labelling PPM1D430/WIP1s with ab234439 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in MCF7 cells. Tubulin is detected with Anti-alpha Tubulin antibody (Alexa Fluor® 594) ab195889 at 1/200 dilution (Red). Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Flow Cytometry - Anti-Secretory stress proteins (SSP) antibody [EPR22960-39] (ab234439)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labelling PPM1D430/WIP1s with ab234439 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-Secretory stress proteins (SSP) antibody [EPR22960-39] (ab234439)

All lanes : Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439) at 1/1000 dilution

Lane 1 : Mouse spleen tissue lysate
Lane 2 : Rat spleen tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Observed band size: 50 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Immunocytochemistry/ Immunofluorescence - Anti-Secretory stress proteins (SSP) antibody [EPR22960-39] (ab234439)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HCT-116 (Human colorectal carcinoma cell line) cells labelling PPM1D430/WIP1s with ab234439 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HCT-116 cells. Tubulin is detected with Anti-alpha Tubulin antibody (Alexa Fluor® 594) ab195889 at 1/200 dilution (Red). Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Western blot - Anti-Secretory stress proteins (SSP) antibody [EPR22960-39] (ab234439)

All lanes : Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439) at 1/1000 dilution

Lane 1 : Human testis tissue lysate
Lane 2 : Human lung cancer tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody (HRP))

Observed band size: 50 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST Exposure time: 3 minutes
Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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