Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IDH1 antibody [HMab-2] - BSA and Azide free (ab256563)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [HMab-2] to IDH1 - BSA and Azide free
  • Suitable for: WB, ELISA
  • Reacts with: Human

Overview

  • Product name

    Anti-IDH1 antibody [HMab-2] - BSA and Azide free
    See all IDH1 primary antibodies

  • Description

    Mouse monoclonal [HMab-2] to IDH1 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged human IDH1 mutated R132H (aa1-414) recombinant protein.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab256563 is the carrier-free version of ab256558. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-IDH1 antibody [HMab-2] - BSA and Azide free (ab256563)
    Western blot - Anti-IDH1 antibody [HMab-2] - BSA and Azide free (ab256563)

    ab256558 used at 1.263µg/ml.
    Goat Anti-Rat IgG (H+L), HRP) (ab205720) used as the secondary at a 1/10,000 dilution.
    Blocking/Dilution buffer:5% NFDM/TBST.

    Lane 1: His-tagged human IDH1 (aa1-414) recombinant protein 10ng
    Lane 2: His-tagged human IDH1 mutated R132H (aa1-414) recombinant protein 10ng
    Lane 3: His-tagged human IDH1 mutated R132G (aa1-414) recombinant protein 10ng
    Lane 4: His-tagged human IDH1 mutated R132L (aa1-414) recombinant protein 10ng
    Lane 5: His-tagged human IDH1 mutated R132S (aa1-414) recombinant protein 10ng
    Lane 6: His-tagged human IDH1 mutated R132V (aa1-414) recombinant protein 10ng
    Lane 7: His-tagged human IDH1 mutated R132C (aa1-414) recombinant protein 10ng
    Lane 8: His-tagged human IDH2 (aa40-452) recombinant protein 10ng
    Lane 9: His-tagged human IDH2 mutated R172M (aa40-452) recombinant protein 10ng
    Lane 10: His-tagged human IDH2 mutated R172S (aa40-452) recombinant protein 10ng
    Lane 11: His-tagged human IDH2 mutated R172G (aa40-452) recombinant protein 10ng
    Lane 12: His-tagged human IDH2 mutated R172W (aa40-452) recombinant protein 10ng
    Lane 13: His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein 10ng
    Lane 14: His-tagged human IDH2 mutated R140Q (aa40-452) recombinant protein 10ng

    The loading samples are E.coil extracts containing recombinant protein respectively.
    Exposure time of 3 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256558).

  • ELISA - Anti-IDH1 antibody [HMab-2] - BSA and Azide free (ab256563)
    ELISA - Anti-IDH1 antibody [HMab-2] - BSA and Azide free (ab256563)

    ELISA - Anti-IDH1 antibody [HMab-2].

    ab256558 used at 0-5000 ng/ml.

    Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) was used as the secondary at a 1/1000 dilution.

    Antigen concentration 1000 ng/ml.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256558).

  • Anti-IDH1 antibody [HMab-2] - BSA and Azide free (ab256563)
    Anti-IDH1 antibody [HMab-2] - BSA and Azide free (ab256563)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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