Anti-PFKP antibody [OTI1D6] (ab119796)
Key features and details
- Mouse monoclonal [OTI1D6] to PFKP
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Dog, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-PFKP antibody [OTI1D6]
See all PFKP primary antibodies -
Description
Mouse monoclonal [OTI1D6] to PFKP -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF African green monkeyIHC-P HumanWB Human -
Immunogen
Recombinant full length Human PFKP produced in HEK293T cells (NP_002618).
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Positive control
- HEK293T cell lysate transfected with pCMV6-ENTRY PFKP; SVT2, A549, Jurkat, PC12 and MCF7 cell extracts; Human colon adenocarcinoma, kidney carcinoma, lung carcinoma, ovary adenocarcinoma, endometrium, endometrium adenocarcinoma, bladder carcinoma and tonsil tissues; COS7 cells transiently transfected with pCMV6-ENTRY PFKP; Jurkat cells.
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General notes
The clone number has been updated from 1D6 to OTI1D6, both clone numbers name the same clone.
Dilute in PBS (pH 7.3) before use.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
OTI1D6 -
Isotype
IgG1 -
Research areas
Images
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All lanes : Anti-PFKP antibody [OTI1D6] (ab119796) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PFKP knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 86 kDa
Observed band size: 86 kDaLanes 1-3: Merged signal (red and green). Green - ab119796 observed at 86 kDa. Red - loading control ab181602 observed at 36 kDa.
ab119796 Anti-PFKP antibody [OTI1D6] was shown to specifically react with PFKP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265136 (knockout cell lysate ab257580) was used. Wild-type and PFKP knockout samples were subjected to SDS-PAGE. ab119796 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Colon tissue by Immunohistochemistry.
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All lanes : Anti-PFKP antibody [OTI1D6] (ab119796) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HeLa cell extract
Lane 3 : SVT2 cell extract
Lane 4 : A549 cell extract
Lane 5 : COS7 cell extract
Lane 6 : Jurkat cell extract
Lane 7 : MDCK cell extract
Lane 8 : PC12 cell extract
Lane 9 : MCF7 cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 86 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200673) -
All lanes : Anti-PFKP antibody [OTI1D6] (ab119796) at 1/500 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PFKP cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 86 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200673) -
ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Kidney carcinoma tissue by Immunohistochemistry.
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ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Lung carcinoma tissue by Immunohistochemistry.
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ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Ovary adenocarcinoma tissue by Immunohistochemistry.
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ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Endometrium tissue by Immunohistochemistry.
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ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Endometrium adenocarcinoma tissue by Immunohistochemistry.
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ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Bladder carcinoma tissue by Immunohistochemistry.
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ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.
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ab119796 at 1/100 dilution staining PFKP in COS7 cells transiently transfected with pCMV6-ENTRY PFKP by Immunofluorescence.
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ab119796 at 1/100 dilution staining PFKP in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).