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Signal Transduction Metabolism Energy Metabolism

Anti-PFKP antibody [OTI1D6] (ab119796)

Price and availability

268 032 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-PFKP antibody [OTI1D6] (ab119796)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [OTI1D6] to PFKP
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Dog, Human, African green monkey
  • Isotype: IgG1

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Overview

  • Product name

    Anti-PFKP antibody [OTI1D6]
    See all PFKP primary antibodies
  • Description

    Mouse monoclonal [OTI1D6] to PFKP
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    African green monkey
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length Human PFKP produced in HEK293T cells (NP_002618).

  • Positive control

    • HEK293T cell lysate transfected with pCMV6-ENTRY PFKP; SVT2, A549, Jurkat, PC12 and MCF7 cell extracts; Human colon adenocarcinoma, kidney carcinoma, lung carcinoma, ovary adenocarcinoma, endometrium, endometrium adenocarcinoma, bladder carcinoma and tonsil tissues; COS7 cells transiently transfected with pCMV6-ENTRY PFKP; Jurkat cells.
  • General notes

    The clone number has been updated from 1D6 to OTI1D6, both clone numbers name the same clone.

    Dilute in PBS (pH 7.3) before use.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from TCS
  • Clonality

    Monoclonal
  • Clone number

    OTI1D6
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Western blot - Anti-PFKP antibody [OTI1D6] (ab119796)
    Western blot - Anti-PFKP antibody [OTI1D6] (ab119796)
    All lanes : Anti-PFKP antibody [OTI1D6] (ab119796) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PFKP knockout HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution

    Predicted band size: 86 kDa
    Observed band size: 86 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab119796 observed at 86 kDa. Red - loading control ab181602 observed at 36 kDa.

    ab119796 Anti-PFKP antibody [OTI1D6] was shown to specifically react with PFKP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265136 (knockout cell lysate ab257580) was used. Wild-type and PFKP knockout samples were subjected to SDS-PAGE. ab119796 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody [OTI1D6] (ab119796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody [OTI1D6] (ab119796)
    ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Colon tissue by Immunohistochemistry.
  • Western blot - Anti-PFKP antibody [OTI1D6] (ab119796)
    Western blot - Anti-PFKP antibody [OTI1D6] (ab119796)
    All lanes : Anti-PFKP antibody [OTI1D6] (ab119796) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 86 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200673)
  • Western blot - Anti-PFKP antibody [OTI1D6] (ab119796)
    Western blot - Anti-PFKP antibody [OTI1D6] (ab119796)
    All lanes : Anti-PFKP antibody [OTI1D6] (ab119796) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PFKP cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 86 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200673)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody [OTI1D6] (ab119796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody [OTI1D6] (ab119796)
    ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Kidney carcinoma tissue by Immunohistochemistry.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody [OTI1D6] (ab119796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody [OTI1D6] (ab119796)
    ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Lung carcinoma tissue by Immunohistochemistry.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody [OTI1D6] (ab119796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody [OTI1D6] (ab119796)
    ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Ovary adenocarcinoma tissue by Immunohistochemistry.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody [OTI1D6] (ab119796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody [OTI1D6] (ab119796)
    ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Endometrium tissue by Immunohistochemistry.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody [OTI1D6] (ab119796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody [OTI1D6] (ab119796)
    ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Endometrium adenocarcinoma tissue by Immunohistochemistry.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody [OTI1D6] (ab119796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody [OTI1D6] (ab119796)
    ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Bladder carcinoma tissue by Immunohistochemistry.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody [OTI1D6] (ab119796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody [OTI1D6] (ab119796)
    ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.
  • Immunocytochemistry/ Immunofluorescence - Anti-PFKP antibody [OTI1D6] (ab119796)
    Immunocytochemistry/ Immunofluorescence - Anti-PFKP antibody [OTI1D6] (ab119796)
    ab119796 at 1/100 dilution staining PFKP in COS7 cells transiently transfected with pCMV6-ENTRY PFKP by Immunofluorescence.
  • Flow Cytometry - Anti-PFKP antibody [OTI1D6] (ab119796)
    Flow Cytometry - Anti-PFKP antibody [OTI1D6] (ab119796)
    ab119796 at 1/100 dilution staining PFKP in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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