Anti-UGPase antibody [EPR10627(B)] (ab154817)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10627(B)] to UGPase
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Human
Overview
-
Product name
Anti-UGPase antibody [EPR10627(B)]
See all UGPase primary antibodies -
Description
Rabbit monoclonal [EPR10627(B)] to UGPase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseIHC-P HumanWB Human -
Immunogen
Synthetic peptide corresponding to Human UGPase (internal sequence).
Database link: Q16851 -
Positive control
- HepG2; Hela; NIH 3T3 cell lysates; Human fetal liver tissue lysate; Human colon adenocarcinoma; kidney and NIH 3T3 cells.
-
General notes
Previously labelled as UGP2.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10627(B) -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-UGPase antibody [EPR10627(B)] (ab154817) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : NIH 3T3 cell lysate
Lane 4 : Human fetal liver tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 57 kDa
-
Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma labeling UGPase using ab154817 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded Human kidney labeling UGPase using ab154817 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Flow cytometric analysis of permeabilized NIH 3T3 cells labeling UGPase using ab154817 (red) at 1/10 dilution or a rabbit IgG (negative) (green).
-
Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154817 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab154817 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded Human normal heart tissue using ab154817 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab154817 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Lanes 1-2 : Anti-UGPase antibody [EPR10627(B)] (ab154817) at 1/10000 dilution
Lanes 3-4 : Anti-UGPase antibody [EPR10627(B)] (ab154817) at 1/20000 dilution
All lanes : Pig muscle sarcoplasm tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG (H+L) polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 45-47 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
-