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Signal Transduction Metabolism Energy Metabolism

Anti-UGPase antibody [EPR10627(B)] (ab154817)

Price and availability

328 339 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-UGPase antibody [EPR10627(B)] (ab154817)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10627(B)] to UGPase
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-UGPase antibody [EPR10627(B)]
    See all UGPase primary antibodies
  • Description

    Rabbit monoclonal [EPR10627(B)] to UGPase
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human UGPase (internal sequence).
    Database link: Q16851

  • Positive control

    • HepG2; Hela; NIH 3T3 cell lysates; Human fetal liver tissue lysate; Human colon adenocarcinoma; kidney and NIH 3T3 cells.
  • General notes

     

     

    Previously labelled as UGP2. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10627(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Images

  • Western blot - Anti-UGPase antibody [EPR10627(B)] (ab154817)
    Western blot - Anti-UGPase antibody [EPR10627(B)] (ab154817)
    All lanes : Anti-UGPase antibody [EPR10627(B)] (ab154817) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : NIH 3T3 cell lysate
    Lane 4 : Human fetal liver tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 57 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] (ab154817)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] (ab154817)

    Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma labeling UGPase using ab154817 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] (ab154817)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] (ab154817)

    Immunohistochemical analysis of paraffin-embedded Human kidney labeling UGPase using ab154817 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-UGPase antibody [EPR10627(B)] (ab154817)
    Flow Cytometry - Anti-UGPase antibody [EPR10627(B)] (ab154817)

    Flow cytometric analysis of permeabilized NIH 3T3 cells labeling UGPase using ab154817 (red) at 1/10 dilution or a rabbit IgG (negative) (green).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] (ab154817)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] (ab154817)

    Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154817 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] (ab154817)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] (ab154817)

    Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab154817 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] (ab154817)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] (ab154817)

    Immunohistochemical analysis of paraffin embedded Human normal heart tissue using ab154817 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] (ab154817)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] (ab154817)

    Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab154817 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-UGPase antibody [EPR10627(B)] (ab154817)
    Western blot - Anti-UGPase antibody [EPR10627(B)] (ab154817) This image is courtesy of an Abreview submitted by Shannon Cruzen
    Lanes 1-2 : Anti-UGPase antibody [EPR10627(B)] (ab154817) at 1/10000 dilution
    Lanes 3-4 : Anti-UGPase antibody [EPR10627(B)] (ab154817) at 1/20000 dilution


    All lanes : Pig muscle sarcoplasm tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG (H+L) polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 45-47 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes

    See Abreview

  • Anti-UGPase antibody [EPR10627(B)] (ab154817)
    Anti-UGPase antibody [EPR10627(B)] (ab154817)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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