Anti-Glucose Transporter GLUT2 antibody (ab54460)
Key features and details
- Rabbit polyclonal to Glucose Transporter GLUT2
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Glucose Transporter GLUT2 antibody
See all Glucose Transporter GLUT2 primary antibodies -
Description
Rabbit polyclonal to Glucose Transporter GLUT2 -
Host species
Rabbit -
Specificity
This antibody recognizes the ~53-61 kDa GLUT2 protein. It does not recognize other GLUT isoforms. -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
A synthetic peptide conjugated to KLH corresponding to amino acids in the cytoplasmic domain of rat Glucose Transporter GLUT2.
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General notes
Following initial thaw, aliquot and freeze (-20°C).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT2 antibody (ab54460)ICC/IF image of ab54460 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54460, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
