Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] (ab234440)
![Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] (ab234440)](https://www.abcam.com/ps/products/234/ab234440/Images/ab234440-4-anti-glucose-transporter-glut2-antibody-epr22946-74-flowcytometry-hepg2-human.jpg "Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] (ab234440)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22946-74] to Glucose Transporter GLUT2
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Glucose Transporter GLUT2 antibody [EPR22946-74]
See all Glucose Transporter GLUT2 primary antibodies -
Description
Rabbit monoclonal [EPR22946-74] to Glucose Transporter GLUT2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human liver, Human hepatocellular carcinoma and Human kidney tissues. Flow Cyt: HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22946-74 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] (ab234440)
Flow cytometric analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labelling Glucose Transporter GLUT2 with ab234440 at 1/500 dilution (Red) as compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] (ab234440)](https://www.abcam.com/ps/products/234/ab234440/Images/ab234440-3-anti-glucose-transporter-glut2-antibody-epr22946-74-immunohistochemistry-kidney-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] (ab234440)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Glucose Transporter GLUT2 with ab234440 at 1/500 dilution (1.07 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human renal tubules. The section was incubated with ab234440 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] (ab234440)](https://www.abcam.com/ps/products/234/ab234440/Images/ab234440-2-anti-glucose-transporter-glut2-antibody-epr22946-74-immunohistochemistry-hepatocellular-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] (ab234440)Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Glucose Transporter GLUT2 with ab234440 at 1/500 dilution (1.07 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human hepatocellular carcinoma (PMID/ 30374065). The section was incubated with ab234440 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] (ab234440)](https://www.abcam.com/ps/products/234/ab234440/Images/ab234440-1-anti-glucose-transporter-glut2-antibody-epr22946-74-immunohistochemistry-liver-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] (ab234440)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Glucose Transporter GLUT2 with ab234440 at 1/500 dilution (1.07 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human liver (PMID/ 30374065). The section was incubated with ab234440 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] (ab234440)](https://www.abcam.com/ps/products/234/ab234440/Images/ab234440-5-benefits-of-recombinant-antibodies.png)
Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] (ab234440)
