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Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] - BSA and Azide free (ab260003)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22946-74] to Glucose Transporter GLUT2 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] - BSA and Azide free
    See all Glucose Transporter GLUT2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22946-74] to Glucose Transporter GLUT2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF,IP or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human liver, Human hepatocellular carcinoma and Human kidney tissues. Flow Cyt: HepG2 cells.

  • General notes

    Ab260003 is the carrier-free version of ab234440. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab260003 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Flow Cytometry - Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] - BSA and Azide free (ab260003)
    Flow Cytometry - Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] - BSA and Azide free (ab260003)

    Flow cytometric analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labelling Glucose Transporter GLUT2 with ab234440 at 1/500 dilution (Red) as compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234440).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] - BSA and Azide free (ab260003)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] - BSA and Azide free (ab260003)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Glucose Transporter GLUT2 with ab234440 at 1/500 dilution (1.07 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human renal tubules. The section was incubated with ab234440 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234440).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] - BSA and Azide free (ab260003)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] - BSA and Azide free (ab260003)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Glucose Transporter GLUT2 with ab234440 at 1/500 dilution (1.07 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human hepatocellular carcinoma (PMID/ 30374065). The section was incubated with ab234440 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234440).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] - BSA and Azide free (ab260003)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] - BSA and Azide free (ab260003)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Glucose Transporter GLUT2 with ab234440 at 1/500 dilution (1.07 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human liver (PMID/ 30374065). The section was incubated with ab234440 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234440).

  • Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] - BSA and Azide free (ab260003)
    Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] - BSA and Azide free (ab260003)

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