Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HKDC1 antibody [EPR19835] (ab209846)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19835] to HKDC1
  • Suitable for: WB, IP
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-HKDC1 antibody [EPR19835]
    See all HKDC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19835] to HKDC1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HKDC1 recombinant protein (aa16-458); HeLa and A549 whole cell lysate; human fetal kidney lysate. IP: HeLa whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-HKDC1 antibody [EPR19835] (ab209846)
    Western blot - Anti-HKDC1 antibody [EPR19835] (ab209846)
    All lanes : Anti-HKDC1 antibody [EPR19835] (ab209846) at 1/1000 dilution

    Lane 1 : HKDC1 recombinant protein (aa16-458) 10 ng
    Lane 2 : Hexokinase II recombinant protein (aa16-458) 10 ng
    Lane 3 : Hexokinase I recombinant protein (aa16-458) 10 ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 103 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/dilution buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-HKDC1 antibody [EPR19835] (ab209846)
    Western blot - Anti-HKDC1 antibody [EPR19835] (ab209846)
    All lanes : Anti-HKDC1 antibody [EPR19835] (ab209846) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HKDC1-knockdown HeLa cell lysate
    Lane 3 : Hexokinase I-knockdown HeLa cell lysate
    Lane 4 : Hexokinase II-knockdown HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 103 kDa
    Observed band size: 102 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/dilution buffer and concentration: 5% NFDM/TBST.

    The cell lysates were kindly provided by our collaborator Dr. Hui Jiang, NIBS

  • Western blot - Anti-HKDC1 antibody [EPR19835] (ab209846)
    Western blot - Anti-HKDC1 antibody [EPR19835] (ab209846)
    All lanes : Anti-HKDC1 antibody [EPR19835] (ab209846) at 1/1000 dilution

    Lane 1 : A549 (human lung carcinoma epithelial cell), whole cell lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Developed using the ECL technique.

    Predicted band size: 103 kDa
    Observed band size: 102 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/dilution buffer and concentration: 5% NFDM/TBST.

     

  • Immunoprecipitation - Anti-HKDC1 antibody [EPR19835] (ab209846)
    Immunoprecipitation - Anti-HKDC1 antibody [EPR19835] (ab209846)

    HKDC1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab209846 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab209846 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg (Input).

    Lane 2: ab209846 IP in HeLa whole cell lysate (+).

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209846 in HeLa whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

  • Anti-HKDC1 antibody [EPR19835] (ab209846)
    Anti-HKDC1 antibody [EPR19835] (ab209846)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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