FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)
![FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)](https://www.abcam.com/ps/products/122/ab122979/Images/Hepatitis-C-Virus-Primary-antibodies-ab122979-3.jpg "FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)")
Key features and details
- FITC Mouse monoclonal [NS5B-6] to Hepatitis C Virus
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Hepatitis C virus
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Overview
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Product name
FITC Anti-Hepatitis C Virus antibody [NS5B-6]
See all Hepatitis C Virus primary antibodies -
Description
FITC Mouse monoclonal [NS5B-6] to Hepatitis C Virus -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
Specific to Human HCV NS5b protein of genotype 1b. Not tested in other genotypes. -
Tested applications
Suitable for: WB, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Hepatitis C virus -
Immunogen
A region of NS5b protein of Hepatitis C Virus genotype 1b expressed in E. coli.
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Positive control
- Chimp liver cells and cell lysate, infected with recombinant vaccinia virus containing a HCV genome cDNA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
ab122979 is filter-sterilized. Purity >90% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
NS5B-6 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Western blot - FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979) + Chimp liver cell lysate, infected with recombinant
vaccinia virus containing a HCV genome cDNA
Predicted band size: 327 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
The NS5b protein is detected as a 58-kDa band. -
![Immunocytochemistry/ Immunofluorescence - FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)](https://www.abcam.com/ps/products/122/ab122979/Images/Hepatitis-C-Virus-Primary-antibodies-ab122979-4.jpg)
Immunocytochemistry/ Immunofluorescence - FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)ab122979, staining Hepatitis C Virus NS5b protein in Acetone-fixed Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA by Immunofluorescence.
