Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972)
![Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972)](https://www.abcam.com/ps/products/122/ab122972/Images/Hepatitis-C-Virus-Primary-antibodies-ab122972-2.jpg "Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972)")
Key features and details
- Biotin Mouse monoclonal [NS5B-6] to Hepatitis C Virus
- Suitable for: WB, ELISA, Flow Cyt, ICC/IF
- Reacts with: Hepatitis C virus
- Conjugation: Biotin
- Isotype: IgG2b
Overview
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Product name
Biotin Anti-Hepatitis C Virus antibody [NS5B-6]
See all Hepatitis C Virus primary antibodies -
Description
Biotin Mouse monoclonal [NS5B-6] to Hepatitis C Virus -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: WB, ELISA, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Hepatitis C virus -
Immunogen
Recombinant fragment of NS5b protein of Hepatitis C Virus genotype 1b, expressed in E. coli
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Positive control
- Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.
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General notes
Conjugate: Biotin conjugated [biotin] / [IgG] = 8.7
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Purification notes
filter-sterilized -
Clonality
Monoclonal -
Clone number
NS5B-6 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Western blot - Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972)Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972) + Lysate of chimp liver cells infected with recombinant
vaccinia virus containing a HCV genome cDNA
Predicted band size: 70 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
NS5b protein is detected as a 58kDa band. -
![Immunocytochemistry/ Immunofluorescence - Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972)](https://www.abcam.com/ps/products/122/ab122972/Images/Hepatitis-C-Virus-Primary-antibodies-ab122972-3.jpg)
Immunocytochemistry/ Immunofluorescence - Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972)ab122972, staining Hepatitis C Virus NS5b protein in Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA by immunofluorescence.
