Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ULBP2 antibody [EPR22303-124] (ab275023)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22303-124] to ULBP2
  • Suitable for: IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-ULBP2 antibody [EPR22303-124]
    See all ULBP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22303-124] to ULBP2

  • Host species

    Rabbit

  • Specificity

    We have preliminary internal testing data to indicate this antibody weakly cross reacts with recombinant human ULBP5 protein in WB. Please contact us for more information.

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: BxPC-3, MOLT-4, HeLa, HCT116 and U-87 MG whole cell lysates. IP: BxPC-3 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22303-124

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ULBP2 antibody [EPR22303-124] (ab275023)
    Western blot - Anti-ULBP2 antibody [EPR22303-124] (ab275023)
    All lanes : Anti-ULBP2 antibody [EPR22303-124] (ab275023) at 1/1000 dilution

    Lane 1 : BxPC-3 (human pancreas adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate
    Lane 3 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
    Lane 4 : Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate
    Lane 5 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 6 : HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate
    Lane 7 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 10 seconds Lane 2-7: 3 minutes.

    Negative control: Raji, Daudi (PMID: 12414645).

  • Immunoprecipitation - Anti-ULBP2 antibody [EPR22303-124] (ab275023)
    Immunoprecipitation - Anti-ULBP2 antibody [EPR22303-124] (ab275023)

    ULBP2 was immunoprecipitated from 0.35 mg BxPC-3 (human pancreas adenocarcinoma epithelial cell), whole cell lysate with ab275023 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275023 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: BxPC-3 whole cell lysate 10 µg

    Lane 2: ab275023 IP in BxPC-3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275023 inBxPC-3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5.5 seconds.

     

  • Anti-ULBP2 antibody [EPR22303-124] (ab275023)
    Anti-ULBP2 antibody [EPR22303-124] (ab275023)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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