Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FOXG1 antibody [mAbcam 51774] (ab51774)

Key features and details

  • Mouse monoclonal [mAbcam 51774] to FOXG1
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-FOXG1 antibody [mAbcam 51774]
    See all FOXG1 primary antibodies

  • Description

    Mouse monoclonal [mAbcam 51774] to FOXG1

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Chicken, Cow, Zebrafish

  • Immunogen

    Synthetic peptide within Human FOXG1 aa 400 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary. Peptide available as ab19644.
    Database link: P55316
    (Peptide available as ab19644)

  • Positive control

    • This antibody gave a positive signal in human brain tissue lysate (ab29466).

  • General notes

    This antibody clone is manufactured by Abcam.

    We can conjugate this antibody to FITC for you (please see ab150200 for details).

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Images

  • Western blot - Anti-FOXG1 antibody [mAbcam 51774] (ab51774)
    Western blot - Anti-FOXG1 antibody [mAbcam 51774] (ab51774)
    Anti-FOXG1 antibody [mAbcam 51774] (ab51774) at 5 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Rabbit polyclonal to Mouse IgG - H&L (HRP) at 1/2000 dilution

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa
    Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.

  • Flow Cytometry - Anti-FOXG1 antibody [mAbcam 51774] (ab51774)
    Flow Cytometry - Anti-FOXG1 antibody [mAbcam 51774] (ab51774)
    Overlay histogram showing SHSY-5Y cells stained with ab51774 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51774, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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