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Signal Transduction Protein Phosphorylation Ser / Thr Phosphatases

Anti-PPP2R2B antibody [EPR10961] (ab157461)

Price and availability

298 185 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-PPP2R2B antibody [EPR10961] (ab157461)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10961] to PPP2R2B
  • Suitable for: WB
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-PPP2R2B antibody [EPR10961]
    See all PPP2R2B primary antibodies
  • Description

    Rabbit monoclonal [EPR10961] to PPP2R2B
  • Host species

    Rabbit
  • Specificity

    The antibody may also recognize PPP2R2A and PPP2R2D based on our internal QC data.
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human PPP2R2B aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HepG2, SH-SY5Y, Jurkat, HeLa and Human fetal brain lysates; Mouse kidney and spleen tissue lysates; rat kidney and spleen tissue lysates
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10961
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Phosphatases
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other

Images

  • Western blot - Anti-PPP2R2B antibody [EPR10961] (ab157461)
    Western blot - Anti-PPP2R2B antibody [EPR10961] (ab157461)
    All lanes : Anti-PPP2R2B antibody [EPR10961] (ab157461) at 1/2000 dilution

    All lanes :

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/2000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 52 kDa
    Observed band size: 52 kDa

  • Western blot - Anti-PPP2R2B antibody [EPR10961] (ab157461)
    Western blot - Anti-PPP2R2B antibody [EPR10961] (ab157461)
    All lanes : Anti-PPP2R2B antibody [EPR10961] (ab157461) at 1/2000 dilution

    Lane 1 : Mouse kidney lysate
    Lane 2 : Mouse spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa

  • Western blot - Anti-PPP2R2B antibody [EPR10961] (ab157461)
    Western blot - Anti-PPP2R2B antibody [EPR10961] (ab157461)
    Anti-PPP2R2B antibody [EPR10961] (ab157461) at 1/1000 dilution + GST-tagged Recombinant Human PPP2R2B protein (aa 1 to 443) 15ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 52 kDa
    Observed band size: 76 kDa
    why is the actual band size different from the predicted?



    Blocking buffer and concentration 5% NFDM/TBST
    Diluting buffer and concentration 5% NFDM /TBST

    Exposure time: 3.25 seconds

    Recombinant Human PPP2R2B protein (Cat#: ab132644)

     

  • Western blot - Anti-PPP2R2B antibody [EPR10961] (ab157461)
    Western blot - Anti-PPP2R2B antibody [EPR10961] (ab157461)
    All lanes : Anti-PPP2R2B antibody [EPR10961] (ab157461) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : Human fetal brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 52 kDa

  • Anti-PPP2R2B antibody [EPR10961] (ab157461)
    Anti-PPP2R2B antibody [EPR10961] (ab157461)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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