Anti-PPP2R2B antibody [EPR10961] (ab157461)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10961] to PPP2R2B
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-PPP2R2B antibody [EPR10961]
See all PPP2R2B primary antibodies -
Description
Rabbit monoclonal [EPR10961] to PPP2R2B -
Host species
Rabbit -
Specificity
The antibody may also recognize PPP2R2A and PPP2R2D based on our internal QC data. -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman -
Immunogen
Synthetic peptide within Human PPP2R2B aa 50-150. The exact sequence is proprietary.
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Positive control
- HepG2, SH-SY5Y, Jurkat, HeLa and Human fetal brain lysates; Mouse kidney and spleen tissue lysates; rat kidney and spleen tissue lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10961 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PPP2R2B antibody [EPR10961] (ab157461) at 1/2000 dilution
All lanes :
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/2000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 52 kDa
Observed band size: 52 kDa
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All lanes : Anti-PPP2R2B antibody [EPR10961] (ab157461) at 1/2000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Mouse spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
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Anti-PPP2R2B antibody [EPR10961] (ab157461) at 1/1000 dilution + GST-tagged Recombinant Human PPP2R2B protein (aa 1 to 443) 15ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted?Blocking buffer and concentration 5% NFDM/TBST
Diluting buffer and concentration 5% NFDM /TBSTExposure time: 3.25 seconds
Recombinant Human PPP2R2B protein (Cat#: ab132644)
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All lanes : Anti-PPP2R2B antibody [EPR10961] (ab157461) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDa
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