Antibodies, Proteins, Kits and Reagents for Life Science

271 382 ₸ Product size

100 µl 271 382 ₸ 10 µl 103 862 ₸

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Anti-ARHGAP27 antibody [EPR12250] (ab171973)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12250] to ARHGAP27
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-ARHGAP27 antibody [EPR12250]
See all ARHGAP27 primary antibodies
Description

Rabbit monoclonal [EPR12250] to ARHGAP27
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment within Human ARHGAP27 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q6ZUM4
Positive control
- HepG2, A549 and BxPC-3 cell lysates; Human breast carcinoma tissue; Human pancreas tissue; BxPC-3 cells and permeabilized A549 cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product was previously labelled as SH3D20

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12250
Isotype

IgG
Research areas

Western blot - Anti-ARHGAP27 antibody [EPR12250] (ab171973)

All lanes : Anti-ARHGAP27 antibody [EPR12250] (ab171973) at 1/1000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : BxPC-3 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 98 kDa

Note: primary antibody diluted in BSA.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP27 antibody [EPR12250] (ab171973)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling ARHGAP27 with ab171973 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP27 antibody [EPR12250] (ab171973)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling ARHGAP27 with ab171973 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP27 antibody [EPR12250] (ab171973)

Immunofluorescent analysis of BxPC-3 cells labeling ARHGAP27 with ab171973 (green) at 1/50 dilution. DAPI nuclear staining (blue).
Flow Cytometry - Anti-ARHGAP27 antibody [EPR12250] (ab171973)

Flow cytometric analysis of permeabilized A549 cells labeling ARHGAP27 with ab171973 at 1/10 dilution (red) or a Rabbit IgG (negative) (green).
Anti-ARHGAP27 antibody [EPR12250] (ab171973)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com