Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ARHGAP27 antibody [EPR12250] (ab171973)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12250] to ARHGAP27
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-ARHGAP27 antibody [EPR12250]
    See all ARHGAP27 primary antibodies

  • Description

    Rabbit monoclonal [EPR12250] to ARHGAP27

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human ARHGAP27 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q6ZUM4

  • Positive control

    • HepG2, A549 and BxPC-3 cell lysates; Human breast carcinoma tissue; Human pancreas tissue; BxPC-3 cells and permeabilized A549 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as SH3D20

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ARHGAP27 antibody [EPR12250] (ab171973)
    Western blot - Anti-ARHGAP27 antibody [EPR12250] (ab171973)
    All lanes : Anti-ARHGAP27 antibody [EPR12250] (ab171973) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : BxPC-3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 98 kDa



    Note: primary antibody diluted in BSA.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP27 antibody [EPR12250] (ab171973)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP27 antibody [EPR12250] (ab171973)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling ARHGAP27 with ab171973 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP27 antibody [EPR12250] (ab171973)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP27 antibody [EPR12250] (ab171973)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling ARHGAP27 with ab171973 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP27 antibody [EPR12250] (ab171973)
    Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP27 antibody [EPR12250] (ab171973)

    Immunofluorescent analysis of BxPC-3 cells labeling ARHGAP27 with ab171973 (green) at 1/50 dilution. DAPI nuclear staining (blue).

  • Flow Cytometry - Anti-ARHGAP27 antibody [EPR12250] (ab171973)
    Flow Cytometry - Anti-ARHGAP27 antibody [EPR12250] (ab171973)

    Flow cytometric analysis of permeabilized A549 cells labeling ARHGAP27 with ab171973 at 1/10 dilution (red) or a Rabbit IgG (negative) (green).

  • Anti-ARHGAP27 antibody [EPR12250] (ab171973)
    Anti-ARHGAP27 antibody [EPR12250] (ab171973)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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