Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PGAM5 antibody [CL0624] (ab244218)

Key features and details

  • Mouse monoclonal [CL0624] to PGAM5
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-PGAM5 antibody [CL0624]
    See all PGAM5 primary antibodies

  • Description

    Mouse monoclonal [CL0624] to PGAM5

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human PGAM5 aa 50-150.
    Database link: Q96HS1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Epitope

    Binds to an epitope located within the peptidesequence NWDRREPLSLINVRK as determined by overlapping synthetic peptides.

  • Positive control

    • IHC-P: Human kidney, liver, testis, uterus, tonsil and small intestine tissues. WB: HeLa, HEPG2, Daudi and RT4 cell lysates.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    CL0624

  • Isotype

    IgG1

  • Research areas

Images

  • Western blot - Anti-PGAM5 antibody [CL0624] (ab244218)
    Western blot - Anti-PGAM5 antibody [CL0624] (ab244218)
    All lanes : Anti-PGAM5 antibody [CL0624] (ab244218)

    Lane 1 : Wild-type HeLa lysate
    Lane 2 : PGAM5 knockout HeLa lysate
    Lane 3 : HepG2 lysate
    Lane 4 : Daudi lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 32 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab244218 observed at 32 kDa. Red - loading control ab52866 observed at 50 kDa.

     ab244218 Anti-PGAM5 antibody [CL0624] was shown to specifically react with PGAM5 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265141 (knockout cell lysate ab257581) was used. Wild-type and PGAM5 knockout samples were subjected to SDS-PAGE. ab244218 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) were incubated overnight at 4°C at 1 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)

    Paraffin-embedded human small intestine tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)

    Paraffin-embedded human tonsil tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)

    Paraffin-embedded human uterus tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)

    Paraffin-embedded human testis tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)

    Paraffin-embedded human liver tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)

    Paraffin-embedded human kidney tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

  • Western blot - Anti-PGAM5 antibody [CL0624] (ab244218)
    Western blot - Anti-PGAM5 antibody [CL0624] (ab244218)
    All lanes : Anti-PGAM5 antibody [CL0624] (ab244218) at 1 µg/ml

    Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate with siRNA control
    Lane 2 : RT4 (human urinary bladder cancer cell line) cell lysate with siRNA probe 1
    Lane 3 : RT4 (human urinary bladder cancer cell line) cell lysate with siRNA probe 2

    Predicted band size: 32 kDa

  • Western blot - Anti-PGAM5 antibody [CL0624] (ab244218)
    Western blot - Anti-PGAM5 antibody [CL0624] (ab244218)
    Anti-PGAM5 antibody [CL0624] (ab244218) at 1 µg + RT4 (human urinary bladder cancer cell line) cell lysate

    Predicted band size: 32 kDa

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