Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Thursday March 04, 2021

Anti-Tin2 antibody [EPR15319] (ab197894)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15319] to Tin2
Suitable for: WB
Reacts with: Mouse, Rat, Human

Product name

Anti-Tin2 antibody [EPR15319]
See all Tin2 primary antibodies
Description

Rabbit monoclonal [EPR15319] to Tin2
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- MCF7, WI38 and HUVEC cell lysate. Mouse brain, heart, kidney and spleen lysate. C6, RAW264.7, PC12 and NIH3T3 cell lysate. Human, mouse and rat thymus lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15319
Isotype

IgG
Research areas

Western blot - Anti-Tin2 antibody [EPR15319] (ab197894)

All lanes : Anti-Tin2 antibody [EPR15319] (ab197894) at 1/10000 dilution

Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : WI38 (Human fetal lung fibroblast cells) whole cell lysate
Lane 3 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 39 kDa
why is the actual band size different from the predicted?

Exposure time: 1 minute

5% NFDM/TBST: Blocking and diluting buffer.
Western blot - Anti-Tin2 antibody [EPR15319] (ab197894)

All lanes : Anti-Tin2 antibody [EPR15319] (ab197894) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : C6 (Rat glial tumor cells) whole cell lysate
Lane 6 : RAW264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 7 : PC12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 8 : NIH3T3 (Mouse embyro fibroblast cells) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

5% NFDM/TBST: Blocking and diluting buffer.
Western blot - Anti-Tin2 antibody [EPR15319] (ab197894)

All lanes : Anti-Tin2 antibody [EPR15319] (ab197894) at 1/2000 dilution

Lane 1 : Human thymus lysate
Lane 2 : Mouse thymus lysate
Lane 3 : Rat thymus lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute

5% NFDM/TBST: Blocking and diluting buffer.
Anti-Tin2 antibody [EPR15319] (ab197894)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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