Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tin2 antibody [EPR15319] (ab197894)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15319] to Tin2
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Tin2 antibody [EPR15319]
    See all Tin2 primary antibodies

  • Description

    Rabbit monoclonal [EPR15319] to Tin2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • MCF7, WI38 and HUVEC cell lysate. Mouse brain, heart, kidney and spleen lysate. C6, RAW264.7, PC12 and NIH3T3 cell lysate. Human, mouse and rat thymus lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Tin2 antibody [EPR15319] (ab197894)
    Western blot - Anti-Tin2 antibody [EPR15319] (ab197894)
    All lanes : Anti-Tin2 antibody [EPR15319] (ab197894) at 1/10000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : WI38 (Human fetal lung fibroblast cells) whole cell lysate
    Lane 3 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 39 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-Tin2 antibody [EPR15319] (ab197894)
    Western blot - Anti-Tin2 antibody [EPR15319] (ab197894)
    All lanes : Anti-Tin2 antibody [EPR15319] (ab197894) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 6 : RAW264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 7 : PC12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 8 : NIH3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 38 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-Tin2 antibody [EPR15319] (ab197894)
    Western blot - Anti-Tin2 antibody [EPR15319] (ab197894)
    All lanes : Anti-Tin2 antibody [EPR15319] (ab197894) at 1/2000 dilution

    Lane 1 : Human thymus lysate
    Lane 2 : Mouse thymus lysate
    Lane 3 : Rat thymus lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 38 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    5% NFDM/TBST: Blocking and diluting buffer.

  • Anti-Tin2 antibody [EPR15319] (ab197894)
    Anti-Tin2 antibody [EPR15319] (ab197894)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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