Anti-Tin2 antibody [EPR15319] - BSA and Azide free (ab239012)
![Anti-Tin2 antibody [EPR15319] - BSA and Azide free (ab239012)](https://www.abcam.com/ps/products/239/ab239012/Images/ab239012-317683-239012.jpg "Anti-Tin2 antibody [EPR15319] - BSA and Azide free (ab239012)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15319] to Tin2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Tin2 antibody [EPR15319] - BSA and Azide free
See all Tin2 primary antibodies -
Description
Rabbit monoclonal [EPR15319] to Tin2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MCF7, WI38 and HUVEC whole cell lysates.
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General notes
Ab239012 is the carrier-free version of ab197894. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab239012 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15319 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Tin2 antibody [EPR15319] - BSA and Azide free (ab239012)All lanes : Anti-Tin2 antibody [EPR15319] (ab197894) at 1/10000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : WI38 (Human fetal lung fibroblast cells) whole cell lysate
Lane 3 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute5% NFDM/TBST: Blocking and diluting buffer.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197894).
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![Anti-Tin2 antibody [EPR15319] - BSA and Azide free (ab239012)](https://www.abcam.com/ps/products/239/ab239012/Images/ab239012-2-benefits-of-recombinant-antibodies.png)
Anti-Tin2 antibody [EPR15319] - BSA and Azide free (ab239012)
