Anti-PURA antibody (ab79936)
Key features and details
- Rabbit polyclonal to PURA
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PURA antibody
See all PURA primary antibodies -
Description
Rabbit polyclonal to PURA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human PURA aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab88160) -
Positive control
- This antibody gave a positive signal in Human Brain Tissue Lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanAll applications MouseSheepApplication Abreviews Notes WB (2) Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa).ICC/IF (1) Use a concentration of 5 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa).ICC/IF
Use a concentration of 5 µg/ml.Target
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Function
This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. May play a role in the initiation of DNA replication and in recombination. -
Sequence similarities
Belongs to the PUR DNA-binding protein family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5813 Human
- Entrez Gene: 19290 Mouse
- Omim: 600473 Human
- SwissProt: Q00577 Human
- SwissProt: P42669 Mouse
- Unigene: 443121 Human
- Unigene: 231802 Mouse
- Unigene: 482241 Mouse
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Alternative names
- PUR ALPHA antibody
- PUR1 antibody
- purA antibody
see all
Images
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Western blot - Anti-PURA antibody (ab79936)Anti-PURA antibody (ab79936) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?PURA contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Furthermore, other commercially available antibodies react with a target at approximately 42 kDa.
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Immunocytochemistry/ Immunofluorescence - Anti-PURA antibody (ab79936)ICC/IF image of ab79936 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79936, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) Hek293. HepG2, MCF-7 cells at 5µg/ml.
Protocols
Datasheets and documents
References (7)
ab79936 has been referenced in 7 publications.
- Swinnen B et al. A zebrafish model for C9orf72 ALS reveals RNA toxicity as a pathogenic mechanism. Acta Neuropathol 135:427-443 (2018). PubMed: 29302778
- Moldovan JB & Moran JV The Zinc-Finger Antiviral Protein ZAP Inhibits LINE and Alu Retrotransposition. PLoS Genet 11:e1005121 (2015). PubMed: 25951186
- Di Salvio M et al. Pur-alpha functionally interacts with FUS carrying ALS-associated mutations. Cell Death Dis 6:e1943 (2015). PubMed: 26492376
- Rossi S et al. Nuclear accumulation of mRNAs underlies G4C2-repeat-induced translational repression in a cellular model of C9orf72 ALS. J Cell Sci 128:1787-99 (2015). PubMed: 25788698
- Hay CW et al. An Sp1 Modulated Regulatory Region Unique to Higher Primates Regulates Human Androgen Receptor Promoter Activity in Prostate Cancer Cells. PLoS One 10:e0139990 (2015). WB . PubMed: 26448047
- Schreiber J et al. Ubiquitin ligase TRIM3 controls hippocampal plasticity and learning by regulating synaptic ?-actin levels. J Cell Biol 211:569-86 (2015). PubMed: 26527743
- Xu Z et al. Expanded GGGGCC repeat RNA associated with amyotrophic lateral sclerosis and frontotemporal dementia causes neurodegeneration. Proc Natl Acad Sci U S A 110:7778-83 (2013). WB . PubMed: 23553836
Images
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Western blot - Anti-PURA antibody (ab79936)Anti-PURA antibody (ab79936) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?PURA contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Furthermore, other commercially available antibodies react with a target at approximately 42 kDa.
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Immunocytochemistry/ Immunofluorescence - Anti-PURA antibody (ab79936)ICC/IF image of ab79936 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79936, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) Hek293. HepG2, MCF-7 cells at 5µg/ml.