Anti-DDX56/DDX21 antibody (ab115158)
Key features and details
- Rabbit polyclonal to DDX56/DDX21
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DDX56/DDX21 antibody
See all DDX56/DDX21 primary antibodies -
Description
Rabbit polyclonal to DDX56/DDX21 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to Human DDX56/DDX21 aa 497-547.
Database link: Q9NY93 -
Positive control
- HeLa and 293T cell lysates.
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General notes
This product was previously labelled as DDX56
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX56/DDX21 antibody (ab115158)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DDX56/DDX21 with ab115158 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-DDX56/DDX21 antibody (ab115158)Immunoprecipitation of DDX56/DDX21 (3 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1-Immunoprecipitation with an anti-DDX56/DDX21 antibody that recognizes an upstream epitope; Lane 2- Immunoprecipitation with anti-DDX56/DDX21 antibody (ab115158); Lane 3- Immunoprecipitation with IgG control. Anti-DDX56/DDX21 antibody (ab115158) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 30 sec.
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Western blot - Anti-DDX56/DDX21 antibody (ab115158)All lanes : Anti-DDX56/DDX21 antibody (ab115158) at 0.1 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Predicted band size: 62 kDa
Exposure time: 3 minutes
