Antibodies, Proteins, Kits and Reagents for Life Science

Anti-BOP1 antibody [6H11] - BSA and Azide free (ab255775)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rat monoclonal [6H11] to BOP1 - BSA and Azide free
Suitable for: WB, ICC, IP
Reacts with: Human

Product name

Anti-BOP1 antibody [6H11] - BSA and Azide free
See all BOP1 primary antibodies
Description

Rat monoclonal [6H11] to BOP1 - BSA and Azide free
Host species

Rat
Tested applications

Suitable for: WB, ICC, IPmore details
Unsuitable for: IHC-P
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Caco-2, HeLa and K562 whole cell lysate. ICC; HeLa and K562 cells. IP: K562 whole cell lysate.
General notes
ab255775 is the carrier-free version of ab252819. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab255775 is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm.

Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

Constituent: 100% PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

6H11
Isotype

IgG1
Research areas

Western blot - Anti-BOP1 antibody [6H11] - BSA and Azide free (ab255775)

All lanes : Anti-BOP1 antibody [6H11] (ab252819) at 1/5000 dilution

Lane 1 : Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

Predicted band size: 84 kDa
Observed band size: 110 kDa
why is the actual band size different from the predicted?

This data was produced using the same clone but in a different formulation, ab252819.

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1: 10 seconds; Lane 2: 59 seconds; Lane 3: 6 seconds.
Immunocytochemistry - Anti-BOP1 antibody [6H11] - BSA and Azide free (ab255775)

This data was produced using the same clone but in a different formulation, ab252819.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling BOP1 with ab252819 at 1/50 dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1: ab252819 at a 1/50 dilution followed by ab150080 at a 1/1000 dilution.

Negative control 2: ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.
Immunoprecipitation - Anti-BOP1 antibody [6H11] - BSA and Azide free (ab255775)

This data was produced using the same clone but in a different formulation, ab252819.

BOP1 was immunoprecipitated from 0.35 mg K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab252819 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab252819 at 1/1,000 dilution. Goat Anti-Rat IgG (H+L), HRP (ab205720) was used at 1/10,000 dilution.

Lane 1: K562 whole cell lysate 10 µg.

Lane 2: ab252819 IP in K562 whole cell lysate.

Lane 3: Rat IgG1 (ab18407) instead of ab252819 in K562 whole cell lysate.

Blocking and dilution buffer and concentration/ 5% NFDM/TBST.

Exposure time: 3 seconds.
Immunocytochemistry - Anti-BOP1 antibody [6H11] - BSA and Azide free (ab255775)

This data was produced using the same clone but in a different formulation, ab252819.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labelling BOP1 with ab252819 at 1/50 dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in K562 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1: ab252819 at a 1/50 dilution followed by ab150080 at a 1/1000 dilution.

Negative control 2: ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.
Anti-BOP1 antibody [6H11] - BSA and Azide free (ab255775)

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