Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RPS16 antibody [EPR11755] (ab177951)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11755] to RPS16
  • Suitable for: ICC/IF, IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RPS16 antibody [EPR11755]
    See all RPS16 primary antibodies

  • Description

    Rabbit monoclonal [EPR11755] to RPS16

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RPS16 aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P62249

  • Positive control

    • MCF-7, fetal liver, HeLa, Ramos, PC-12 and NIH/3T3 cell lysates; Breast carcinoma tissue; MCF-7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11755

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-RPS16 antibody [EPR11755] (ab177951)
    Immunoprecipitation - Anti-RPS16 antibody [EPR11755] (ab177951)

    Western blot analysis on immunoprecipitation pellet from fetal liver lysate using ab177951.

  • Immunocytochemistry/ Immunofluorescence - Anti-RPS16 antibody [EPR11755] (ab177951)
    Immunocytochemistry/ Immunofluorescence - Anti-RPS16 antibody [EPR11755] (ab177951)

    Immunofluorescent analysis of MCF-7 cells labeling RPS16 with ab177951 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS16 antibody [EPR11755] (ab177951)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS16 antibody [EPR11755] (ab177951)

    Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue labeling RPS16 with ab177951 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-RPS16 antibody [EPR11755] (ab177951)
    Western blot - Anti-RPS16 antibody [EPR11755] (ab177951)
    All lanes : Anti-RPS16 antibody [EPR11755] (ab177951) at 1/1000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : Fetal liver lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Ramos cell lysate
    Lane 5 : PC-12 cell lysate
    Lane 6 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 16 kDa

  • Anti-RPS16 antibody [EPR11755] (ab177951)
    Anti-RPS16 antibody [EPR11755] (ab177951)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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