Anti-RPLP0 antibody [EP15646] (ab192866)
![Anti-RPLP0 antibody [EP15646] (ab192866)](https://www.abcam.com/ps/products/192/ab192866/Images/ab192866-230675-anti-rplp0-antibody-ep15646-western-blot.jpg "Anti-RPLP0 antibody [EP15646] (ab192866)")
Key features and details
- Rabbit monoclonal [EP15646] to RPLP0
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-RPLP0 antibody [EP15646]
See all RPLP0 primary antibodies -
Description
Rabbit monoclonal [EP15646] to RPLP0 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseRatHumanWB MouseHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293, HeLa, Jurkat, A375, C6, RAW264.7, PC12 and NIH 3T3 cell lysates; mouse kidney, mouse spleen, rat brain and rat spleen lysates; Human stomach tissue, mouse liver tissue, rat cerebral cortex tissue; HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP15646 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RPLP0 antibody [EP15646] (ab192866)All lanes : Anti-RPLP0 antibody [EP15646] (ab192866) at 1/20000 dilution
Lane 1 : 293 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A375 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
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![Western blot - Anti-RPLP0 antibody [EP15646] (ab192866)](https://www.abcam.com/ps/products/192/ab192866/Images/ab192866-230674-anti-rplp0-antibody-ep15646-western-blot.jpg)
Western blot - Anti-RPLP0 antibody [EP15646] (ab192866)All lanes : Anti-RPLP0 antibody [EP15646] (ab192866) at 1/1000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat spleen lysate
Lane 5 : C6 cell lysate
Lane 6 : RAW264.7 cell lysate
Lane 7 : PC12 cell lysate
Lane 8 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (ab192866)](https://www.abcam.com/ps/products/192/ab192866/Images/ab192866-230673-anti-rplp0-antibody-ep15646-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (ab192866)Immunohistochemical analysis of paraffin embedded Human stomach tissue sections labeling RPLP0 using ab192866 at a 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (ab192866)](https://www.abcam.com/ps/products/192/ab192866/Images/ab192866-230672-anti-rplp0-antibody-ep15646-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (ab192866)Immunohistochemical analysis of paraffin embedded rmouse liver tissue sections labeling RPLP0 using ab192866 at a 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (ab192866)](https://www.abcam.com/ps/products/192/ab192866/Images/ab192866-230671-anti-rplp0-antibody-ep15646-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (ab192866)Immunohistochemical analysis of paraffin embedded rat cerebral cortex tissue sections labeling RPLP0 using ab192866 at a 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-RPLP0 antibody [EP15646] (ab192866)](https://www.abcam.com/ps/products/192/ab192866/Images/ab192866-230670-anti-rplp0-antibody-ep15646-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RPLP0 antibody [EP15646] (ab192866)Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling RPLP0 with ab192866 at a 1/1000 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.
