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Epigenetics and Nuclear Signaling DNA / RNA Translation Ribosome

Anti-FRG1 antibody [EPR13098] (ab181083)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-FRG1 antibody [EPR13098] (ab181083)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13098] to FRG1
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-FRG1 antibody [EPR13098]
    See all FRG1 primary antibodies
  • Description

    Rabbit monoclonal [EPR13098] to FRG1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, Jurkat, HEK293 whole cell lysate; HeLa cells
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13098
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing

Images

  • Western blot - Anti-FRG1 antibody [EPR13098] (ab181083)
    Western blot - Anti-FRG1 antibody [EPR13098] (ab181083)
    All lanes : Anti-FRG1 antibody [EPR13098] (ab181083) at 1/10000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : Jurkat whole cell lysate
    Lane 3 : 293 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 29 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-FRG1 antibody [EPR13098] (ab181083)
    Immunocytochemistry/ Immunofluorescence - Anti-FRG1 antibody [EPR13098] (ab181083)

    Immunofluorescent analysis of HeLa cells labeling FRG1 with ab181083 at a 1/100 dilution. Primary incubation was followed by secondary antibody labeling with Alexa Fluor 555-conjugated goat anti-rabbit lgG (red - left panel). DAPI was used to stain the cell nuclear (blue - right panel).

  • Immunoprecipitation - Anti-FRG1 antibody [EPR13098] (ab181083)
    Immunoprecipitation - Anti-FRG1 antibody [EPR13098] (ab181083)

    Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate labeling FRG1 with ab181083 at 1/30 dilution. Right lane is the negative control.

  • Anti-FRG1 antibody [EPR13098] (ab181083)
    Anti-FRG1 antibody [EPR13098] (ab181083)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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