Anti-FRG1 antibody [EPR13098] (ab181083)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13098] to FRG1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Overview
-
Product name
Anti-FRG1 antibody [EPR13098]
See all FRG1 primary antibodies -
Description
Rabbit monoclonal [EPR13098] to FRG1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIP HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HeLa, Jurkat, HEK293 whole cell lysate; HeLa cells
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13098 -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-FRG1 antibody [EPR13098] (ab181083) at 1/10000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : 293 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 29 kDa
-
Immunofluorescent analysis of HeLa cells labeling FRG1 with ab181083 at a 1/100 dilution. Primary incubation was followed by secondary antibody labeling with Alexa Fluor 555-conjugated goat anti-rabbit lgG (red - left panel). DAPI was used to stain the cell nuclear (blue - right panel).
-
Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate labeling FRG1 with ab181083 at 1/30 dilution. Right lane is the negative control.
-