Anti-alpha Internexin antibody (ab226374)
Key features and details
- Rabbit polyclonal to alpha Internexin
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-alpha Internexin antibody
See all alpha Internexin primary antibodies -
Description
Rabbit polyclonal to alpha Internexin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human alpha Internexin aa 150-200. The exact sequence is proprietary.
Database link: Q16352 -
Positive control
- IP: HEK-293T whole cell lysate.
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General notes
ab226374 has not performed satisfactorily when used for WB of alpha Internexin in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of alpha Internexin.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226374 was affinity purified using an epitope specific to alpha Internexin immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-alpha Internexin antibody (ab226374)
alpha Internexin was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226374 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226374 at 0.4 µg/ml.
Lane 1: ab226374 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
