Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Tuesday March 02, 2021

Anti-alpha Internexin antibody [EP676Y] (ab40758)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP676Y] to alpha Internexin
Suitable for: WB, IHC-P, ICC/IF
Knockout validated
Reacts with: Rat, Human

Product name

Anti-alpha Internexin antibody [EP676Y]
See all alpha Internexin primary antibodies
Description

Rabbit monoclonal [EP676Y] to alpha Internexin
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Rat
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human alpha Internexin aa 400-500. The exact sequence is proprietary.
Positive control
- HeLa, SHSY5Y cell lysate, human brain lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP676Y
Isotype

IgG
Research areas

Western blot - Anti-alpha Internexin antibody [EP676Y] (ab40758)

All lanes : Anti-alpha Internexin antibody [EP676Y] (ab40758) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : INA knockout HeLa cell lysate
Lane 3 : SH-SY5Y cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 66 kDa
Observed band size: 66 kDa

Lanes 1-4: Merged signal (red and green). Green - ab40758 observed at 66 kDa. Red - loading control ab8245 observed at 36 kDa.

ab40758 Anti-alpha Internexin antibody [EP676Y] was shown to specifically react with alpha Internexin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265264 (knockout cell lysate ab257482) was used. Wild-type and alpha Internexin knockout samples were subjected to SDS-PAGE. ab40758 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-alpha Internexin antibody [EP676Y] (ab40758)

Anti-alpha Internexin antibody [EP676Y] (ab40758) at 1/10000 dilution + SHSY5Y cell lysate at 10 µg

Predicted band size: 66 kDa
Observed band size: 60 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EP676Y] (ab40758)

Positive staining of alpha Internexin on paraffin-embedded human brain tissue (A) using ab40758 diluited 1:250. Negative staining of paraffin-embedded human colon (B), Kidney (C) and uterus (D) tissues using ab40758 under the same conditions.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-alpha Internexin antibody [EP676Y] (ab40758) This image is courtesy of an anonymous Abreview

ab40758 staining alpha Internexin in rat glial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 1% BSA for 10 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 10 hours at 4°C. An undiluted Alexa Fluor^® 594-conjugated donkey anti-rabbit IgG polyclonal was used as the secondary antibody.
See Abreview
Immunocytochemistry/ Immunofluorescence - Anti-alpha Internexin antibody [EP676Y] (ab40758)

Immunofluorescent staining of alpha Internexin on PC 12 cells using ab40758 diluted 1:250.
Anti-alpha Internexin antibody [EP676Y] (ab40758)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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