Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-alpha Internexin antibody [2E3] (ab7654)

Key features and details

  • Mouse monoclonal [2E3] to alpha Internexin
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-alpha Internexin antibody [2E3]
    See all alpha Internexin primary antibodies

  • Description

    Mouse monoclonal [2E3] to alpha Internexin

  • Host species

    Mouse

  • Specificity

    specifically recognizes a-internexin.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Rat
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Rat alpha Internexin. (Purified from E. coli).

  • Epitope

    C-terminal non-helical extension of the protein.

  • Positive control

    • WB: Rat and mouse brain and spinal cord lysates; Cow spinal cord lysate. Flow Cyt: SH-SY5Y cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-alpha Internexin antibody [2E3] (ab7654)
    Western blot - Anti-alpha Internexin antibody [2E3] (ab7654)
    All lanes : Anti-alpha Internexin antibody [2E3] (ab7654) at 1/10000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat spinal cord lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Mouse spinal cord lysate
    Lane 5 : Cow spinal cord lysate

    Predicted band size: 66 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Internexin antibody [2E3] (ab7654)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Internexin antibody [2E3] (ab7654)
    Hippocampal neurons in tissue culture with neurofilament alpha internexin (red) (Ab7654) and GFAP (green). Photo courtesy of: Dr. Gerry Shaw University of Florida
  • Western blot - Anti-alpha Internexin antibody [2E3] (ab7654)
    Western blot - Anti-alpha Internexin antibody [2E3] (ab7654)
    All lanes : Anti-alpha Internexin antibody [2E3] (ab7654)

    Lanes 1-2 : Crude homogenate of rat facial nucleus at 3 days after experimental injury
    Lanes 3-4 : Crude homogenate of rat facial nucleus at 5 days after experimental injury
    Lanes 5-6 : Crude homogenate of rat facial nucleus at 7 days after experimental injury
    Lanes 7-8 : Crude homogenate of rat facial nucleus at 14 days after experimental injury
    Lanes 9-10 : Crude homogenate of rat facial nucleus at 28 days after experimental injury

    Predicted band size: 66 kDa
    Observed band size: 66 kDa

  • Flow Cytometry - Anti-alpha Internexin antibody [2E3] (ab7654)
    Flow Cytometry - Anti-alpha Internexin antibody [2E3] (ab7654)
    Overlay histogram showing SH-SY5Y cells stained with ab7654 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7654, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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