Anti-Nestin antibody [196908] (ab6320)
![Anti-Nestin antibody [196908] (ab6320)](https://www.abcam.com/ps/products/6/ab6320/Images/ab6320-333750-anti-nestin-antibody-196908-western-blot.jpg "Anti-Nestin antibody [196908] (ab6320)")
Key features and details
- Mouse monoclonal [196908] to Nestin
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Nestin antibody [196908]
See all Nestin primary antibodies -
Description
Mouse monoclonal [196908] to Nestin -
Host species
Mouse -
Specificity
Based on the result of immunocytochemistry on mouse and rat neural stem cells, this antibody has no reactivity with rodent Nestin. The clone number has been updated from (3k1) to (196908) both clone numbers name the same antibody clone. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Nestin-transfected NS0 cells transfected with a human Nestin fragment aa residues 618-1618.
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Positive control
- Human neural progenitors and A172 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituents: PBS, 5% Trehalose -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
196908 -
Myeloma
NS0 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Nestin antibody [196908] (ab6320)All lanes : Anti-Nestin antibody [196908] (ab6320) at 1 µg
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : NES knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 177 kDaLanes 1 - 2: Merged signal (red and green). Green - ab6320 observed at 300 kDa. Red - loading control, ab176560, observed at 50 kDa.
ab6320 was shown to recognize NES in wild-type HAP1 cells as signal was lost at the expected MW in NES knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and NES knockout samples were subjected to SDS-PAGE. Ab6320 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [196908] (ab6320)](https://www.abcam.com/ps/products/6/ab6320/Images/ab6320-276469-anti-nestin-antibody-196908-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [196908] (ab6320)Immunocytochemical analysis of human fetal neural progenitor cells, labeling Nestin with ab6320 (10µg/ml). Immunostaining was for 3 hours at room temperature. Counterstaining with DAPI (blue).
