All lanes : Anti-alpha Internexin antibody (ab227614) at 1/1000 dilution

Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell lysate
Lane 3 : SK-N-AS whole cell lysate

Lysates/proteins at 30 µg per lane.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 55 kDa

10% SDS-PAGE

Rat E18 primary cortical neuron DIV 8 cells stained for alpha Internexin (red) using ab227614 at 1/250 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Blue: Fluoroshield with DAPI.

Anti-alpha Internexin antibody (ab227614) at 1/1000 dilution + SK-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate at 30 µg

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 55 kDa

10% SDS-PAGE

Anti-alpha Internexin antibody (ab227614) at 1/1000 dilution + Mouse brain tissue lysate at 50 µg

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 55 kDa

10% SDS-PAGE

Anti-alpha Internexin antibody (ab227614) at 1/1000 dilution + Rat brain tissue lysate at 50 µg

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 55 kDa

10% SDS-PAGE

alpha Internexin was immunoprecipitated from U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate with 5 μg ab227614. Western blot was performed from the immunoprecipitate using ab227614. Anti-rabbit IgG was used as secondary antibody.

Lane 1: IgG instead of ab227614 in U-87 MG whole cell lysate

Lane 2: ab227614 IP in U-87 MG whole cell lysate.