HRP Anti-Histone H2B antibody [mAbcam 52484] (ab204463)
![HRP Anti-Histone H2B antibody [mAbcam 52484] (ab204463)](https://www.abcam.com/ps/products/204/ab204463/Images/ab204463-265651-anti-histone-h2b-antibody-mabcam-52484-hrp-western-blot.jpg "HRP Anti-Histone H2B antibody [mAbcam 52484] (ab204463)")
Key features and details
- HRP Mouse monoclonal [mAbcam 52484] to Histone H2B
- Suitable for: IHC-P, WB
- Reacts with: Human, Recombinant fragment
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-Histone H2B antibody [mAbcam 52484]
See all Histone H2B primary antibodies -
Description
HRP Mouse monoclonal [mAbcam 52484] to Histone H2B -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Recombinant fragment
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Immunogen
Synthetic peptide corresponding to Human Histone H2B aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
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Positive control
- WB: Histone H2B Recombinant Protein. IHC-P: FFPE human colon (normal) tissue sections.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
mAbcam 52484 -
Myeloma
Sp2/0 -
Isotype
IgG1
Images
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Western blot - HRP Anti-Histone H2B antibody [mAbcam 52484] (ab204463)All lanes : HRP Anti-Histone H2B antibody [mAbcam 52484] (ab204463) at 1/7500 dilution
Lane 1 : Histone H1 Recombinant Protein
Lane 2 : Histone H2A Recombinant Protein
Lane 3 : Histone H2B Recombinant Protein
Lane 4 : Histone H3 Recombinant Protein
Lane 5 : Histone H4 Recombinant Protein
Lysates/proteins at 0.1 µg per lane.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab204463 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Histone H2B antibody [mAbcam 52484] (ab204463)](https://www.abcam.com/ps/products/204/ab204463/Images/ab204463-253093-anti-histone-h2b-antibody-mabcam-52484-hrp-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Histone H2B antibody [mAbcam 52484] (ab204463)IHC image of Histone H2B staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab204463, 1/1000 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
