Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TSPAN17 antibody [EPR9855(2)] - BSA and Azide free (ab250243)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9855(2)] to TSPAN17 - BSA and Azide free
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TSPAN17 antibody [EPR9855(2)] - BSA and Azide free
    See all TSPAN17 primary antibodies

  • Description

    Rabbit monoclonal [EPR9855(2)] to TSPAN17 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250243 is the carrier-free version of ab180601. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250243 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Immunoprecipitation - Anti-TSPAN17 antibody [EPR9855(2)] - BSA and Azide free (ab250243)
    Immunoprecipitation - Anti-TSPAN17 antibody [EPR9855(2)] - BSA and Azide free (ab250243)
    This data was developed using ab180601, the same antibody clone in a different buffer formulation.Western blot analysis of Jurkat cell lysate immuoprecipitated with ab180601 at 1/60 dilution. Goat Anti-Rabbit IgG, (H+L) peroxidase conjugated secondary antibody used at 1/1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPAN17 antibody [EPR9855(2)] - BSA and Azide free (ab250243)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPAN17 antibody [EPR9855(2)] - BSA and Azide free (ab250243)
    This data was developed using ab180601, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human Transitional cell bladder carcinoma tissue labeling TSPAN17 with ab180601 at 1/100 dilution, followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
  • Western blot - Anti-TSPAN17 antibody [EPR9855(2)] - BSA and Azide free (ab250243)
    Western blot - Anti-TSPAN17 antibody [EPR9855(2)] - BSA and Azide free (ab250243)
    All lanes : Anti-TSPAN17 antibody [EPR9855(2)] (ab180601) at 1/20000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Caco-2 cell lysate
    Lane 4 : SW480cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab180601, the same antibody clone in a different buffer formulation.

     

  • Anti-TSPAN17 antibody [EPR9855(2)] - BSA and Azide free (ab250243)
    Anti-TSPAN17 antibody [EPR9855(2)] - BSA and Azide free (ab250243)

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