Anti-ATG9A antibody - C-terminal (ab229334)
Key features and details
- Rabbit polyclonal to ATG9A - C-terminal
- Suitable for: IHC-P, WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ATG9A antibody - C-terminal
See all ATG9A primary antibodies -
Description
Rabbit polyclonal to ATG9A - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig, Rhesus monkey
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Immunogen
Synthetic peptide within Human ATG9A (C terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: Q7Z3C6 -
Positive control
- WB: HeLa, HEK-293T, A431, PC-12, Rat-2, BCL-1 and RD whole cell lysates. IHC-P: Human colon carcinoma tissue. ICC/IF: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ATG9A antibody - C-terminal (ab229334)Anti-ATG9A antibody - C-terminal (ab229334) at 1/1000 dilution + RD (human rhabdomyosarcoma cell line) whole cell extract at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 94 kDa7.5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-ATG9A antibody - C-terminal (ab229334)A431 (human epidermoid carcinoma cell line) cells were stained for ATG9A (green) using ab229334 at 1/500 dilution in ICC/IF. Cells were fixed in 100% methanol for 5 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG9A antibody - C-terminal (ab229334)Paraffin-embedded human colon carcinoma tissue stained for ATG9A using ab229334 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-ATG9A antibody - C-terminal (ab229334)Anti-ATG9A antibody - C-terminal (ab229334) at 1/1000 dilution + BCL-1 (mouse B lymphocyte lymphoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 94 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-ATG9A antibody - C-terminal (ab229334)All lanes : Anti-ATG9A antibody - C-terminal (ab229334) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : Rat2 (rat fibroblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 94 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-ATG9A antibody - C-terminal (ab229334)All lanes : Anti-ATG9A antibody - C-terminal (ab229334) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 94 kDa7.5% SDS-PAGE gel.
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Immunoprecipitation - Anti-ATG9A antibody - C-terminal (ab229334)ATG9A was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 4 μg of ab229334. Western blot was performed from the immunoprecipitate using ab229334 at 1/500 dilution.
Lane 1: 4 μg preimmune rabbit igG instead of ab229334 in HeLa whole cell extract.
Lane 2: ab229334 IP in HeLa whole cell extract.7.5% SDS-PAGE gel.
