Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rb (phospho T373) antibody [EP821Y] - BSA and Azide free (ab219158)

Key features and details

  • Rabbit monoclonal [EP821Y] to Rb (phospho T373) - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, IP, Dot blot, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Rb (phospho T373) antibody [EP821Y] - BSA and Azide free
    See all Rb primary antibodies

  • Description

    Rabbit monoclonal [EP821Y] to Rb (phospho T373) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, IP, Dot blot, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab219158 is the carrier-free version of ab52975 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab219158 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Rb (phospho T373) antibody [EP821Y] - BSA and Azide free (ab219158)
    Immunocytochemistry/ Immunofluorescence - Anti-Rb (phospho T373) antibody [EP821Y] - BSA and Azide free (ab219158)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized MCF7(Human breast denocarcinoma cell line) cells, Serum starved and non-starved, labeling Rb (phospho T373) with Ab52975 at 1/100 dilution followed by Goat anti-Rabbit secondary IgG AlexaFluor®488 (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining on MCF7 cells.The number of positive cells increased after treatment with FBS (fetal bovine serum) for 48 hours, then decreased after Lambda Protein Phosphatase treatment (31? for 2hours ).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52975).

  • Immunoprecipitation - Anti-Rb (phospho T373) antibody [EP821Y] - BSA and Azide free (ab219158)
    Immunoprecipitation - Anti-Rb (phospho T373) antibody [EP821Y] - BSA and Azide free (ab219158)
    All lanes : Anti-Rb (phospho T373) antibody [EP821Y] (ab52975) at 1/500 dilution

    Lane 1 : Jurkat (human acute T cell leukemia) starved 24h then 10% FBS incubated for 20h whole cell lysate.
    Lane 2 : Jurkat (human acute T cell leukemia ) starved 24h then 10% FBS incubated for 20h whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab52975 in Jurkat (human acute T cell leukemia ) starved 24h then 10% FBS incubated for 20h whole cell lysate.

    Lysates/proteins at 10 µg per lane.

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution

    Predicted band size : 106 kDa
    Additional bands at : 110 kDa.

    Exposure time : 1 second

    Blocking and diluting buffer: 5% NFDM/TBST

    The IP lysates are prepared by RIPA method. As the WB image shows, this antibody works in 1%SDS Hot Lysate buffer in WB. So there is no band in input lane.
     
    For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52975).

  • Dot Blot - Anti-Rb (phospho T373) antibody [EP821Y] - BSA and Azide free (ab219158)
    Dot Blot - Anti-Rb (phospho T373) antibody [EP821Y] - BSA and Azide free (ab219158)

    Dot Blot analysis of Lane 1: Rb (pT373) phospho peptide and Lane 2: Rb non-phospho peptide labeling Rb (phospho T373) with ab52975 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.

     

     

     

     

     

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52975).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T373) antibody [EP821Y] - BSA and Azide free (ab219158)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T373) antibody [EP821Y] - BSA and Azide free (ab219158)

    ab52975, at a 1/250 dilution, staining Human Rb (Phosphorylated) in Kidney carcinoma, using Immunohistochemistry, Paraffin Embedded tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52975).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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